Construct: ORF TRCN0000480823
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003293.1_s317c1
- Derived from:
- ccsbBroadEn_03978
- DNA Barcode:
- TGGGACGTTTAGTGTGGTGCTGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ETNPPL (64850)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480823
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64850 | ETNPPL | ethanolamine-phosphate phos... | NM_031279.4 | 100% | 100% | |
| 2 | human | 64850 | ETNPPL | ethanolamine-phosphate phos... | NM_001146590.2 | 98.7% | 98.7% | 174_175insGTGGGACACTGTCACCCA |
| 3 | human | 64850 | ETNPPL | ethanolamine-phosphate phos... | NM_001331031.2 | 91.9% | 91.9% | 0_1ins120 |
| 4 | human | 64850 | ETNPPL | ethanolamine-phosphate phos... | NM_001331032.2 | 91.9% | 91.9% | 0_1ins120 |
| 5 | human | 64850 | ETNPPL | ethanolamine-phosphate phos... | NM_001146627.2 | 88.3% | 88.1% | 1_1delAins175 |
| 6 | human | 64850 | ETNPPL | ethanolamine-phosphate phos... | NM_001331033.2 | 85.3% | 85.3% | 0_1ins219 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1563
- ORF length:
- 1497
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg cgagctgtac agtaagcggg acactctggg gctgaggaag aagcacatcg 121 ggccctcatg caaagttttc tttgcatcgg atcccatcaa aatagtgaga gcccagaggc 181 agtacatgtt tgatgagaac ggtgaacagt acttggactg catcaacaat gttgcccatg 241 tgggacactg tcacccagga gtggtcaaag ctgccctgaa acagatggaa ctgctaaata 301 caaattctcg attcctccac gacaacattg ttgagtatgc caaacgcctt tcagcaactc 361 tgccggagaa actctctgtt tgttatttta caaattcagg atccgaagcc aacgacttag 421 ccttacgcct ggctcggcag ttcagaggcc accaggatgt gatcactctt gaccatgctt 481 accatggtca cctatcatcc ttaattgaga ttagcccata taagtttcag aaaggaaaag 541 atgtcaaaaa agaatttgta catgtggcac caactccaga tacttacaga ggaaaatata 601 gagaagacca tgcagactca gccagtgctt atgcagatga agtgaagaaa atcattgaag 661 atgctcataa cagtggaagg aagattgctg cctttattgc tgaatccatg cagagttgtg 721 gcggacaaat aattcctcca gcaggctact tccagaaagt ggcagaatat gtacacggtg 781 cagggggtgt gtttatagct gatgaagttc aagtgggctt tggcagagtt gggaaacatt 841 tctggagctt ccagatgtat ggtgaagact ttgttccaga catcgtcaca atgggaaaac 901 cgatgggcaa cggccacccg gtggcatgtg tggtaacaac caaagaaatt gcagaagcct 961 tcagcagctc tgggatggaa tattttaata cgtatggagg aaatccagta tcttgtgctg 1021 ttggtttggc tgtcctggat ataattgaaa atgaagacct tcaaggaaat gccaagagag 1081 tagggaatta tctcactgag ttactgaaaa aacagaaggc taaacacact ttgataggag 1141 atattagggg cattGGCCTT TTTATTGGAA TTGATTTAGT GAAGGACCAT CTGAAAAGGA 1201 CCCCTGCCAC AGCTGAAGCT CAGCACATCA TCTACAAGAT GAAAGAAAAA CGAGTGCTTC 1261 TCAGTGCCGA TGGACCTCAT AGAAATGTAC TTAAAATAAA ACCACCTATG TGCTTCACTG 1321 AAGAAGATGC AAAGTTCATG GTGGACCAAC TTGATAGGAT TCTAACAGTT TTAGAAGAAG 1381 CTATGGGAAC CAAAACCGAA AGTGTGACCT CTGAGAATAC TCCATGCAAA ACAAAGATGC 1441 TGAAAGAAGC CCACATAGAA CTGCTTAGGG ACAGCACCAC TGACTCCAAA GAAAATCCCA 1501 GCAGAAAGAG AAATGGAATG TGCACGGATA CACATTCACT GCTCAGTAAG AGGCTCAAGA 1561 CATGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1621 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1681 GGCTTTATAT ATCTTGTGGA AAGGACGATG GGACGTTTAG TGTGGTGCTG CCACGCGTTA 1741 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt