Construct: ORF TRCN0000480843
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011774.1_s317c1
- Derived from:
- ccsbBroadEn_01214
- DNA Barcode:
- TCTAGTCAGAAGACCTATAGCCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLA2G5 (5322)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480843
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5322 | PLA2G5 | phospholipase A2 group V | NM_000929.3 | 100% | 100% | |
| 2 | human | 5322 | PLA2G5 | phospholipase A2 group V | XM_005245893.5 | 100% | 100% | |
| 3 | human | 5322 | PLA2G5 | phospholipase A2 group V | XM_011541587.3 | 100% | 100% | |
| 4 | human | 5322 | PLA2G5 | phospholipase A2 group V | XM_011541588.3 | 100% | 100% | |
| 5 | human | 5322 | PLA2G5 | phospholipase A2 group V | XM_011541589.3 | 100% | 100% | |
| 6 | human | 5322 | PLA2G5 | phospholipase A2 group V | XM_011541590.3 | 100% | 100% | |
| 7 | human | 5322 | PLA2G5 | phospholipase A2 group V | XM_011541591.3 | 100% | 100% | |
| 8 | human | 5322 | PLA2G5 | phospholipase A2 group V | XM_011541592.3 | 100% | 100% | |
| 9 | human | 5322 | PLA2G5 | phospholipase A2 group V | XM_005245891.5 | 81.6% | 81.6% | 1_93del |
| 10 | human | 5322 | PLA2G5 | phospholipase A2 group V | XM_005245892.5 | 81.6% | 81.6% | 1_93del |
| 11 | human | 5322 | PLA2G5 | phospholipase A2 group V | XM_011541586.3 | 81.6% | 81.6% | 1_93del |
| 12 | human | 5322 | PLA2G5 | phospholipase A2 group V | XM_024447706.1 | 63.7% | 44.5% | 185_261del;390_391ins101 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 480
- ORF length:
- 414
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa aggcctcctc ccactggctt ggttcctggc ttgtagtgtg cctgctgtgc 121 aaggaggctt gctggaccta aaatcaatga tcgagaaggt gacagggaag aacgccctga 181 caaactacgg cttctacggc tgtTACTGCG GCTGGGGCGG CCGAGGAACC CCCAAGGATG 241 GCACCGATTG GTGCTGTTGG GCGCATGACC ACTGCTATGG GCGGCTGGAG GAGAAGGGCT 301 GCAACATTCG CACACAGTCC TACAAATACA GATTCGCGTG GGGCGTGGTC ACCTGCGAGC 361 CCGGGCCCTT CTGCCATGTG AACCTCTGTG CCTGTGACCG GAAGCTCGTC TACTGCCTCA 421 AGAGAAACCT ACGGAGCTAC AACCCACAGT ACCAATACTT TCCCAACATC CTCTGCTCCT 481 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 541 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 601 TTTATATATC TTGTGGAAAG GACGATCTAG TCAGAAGACC TATAGCCAGA CGCGTTAAGT 661 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt