Construct: ORF TRCN0000480855
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007359.1_s317c1
- Derived from:
- ccsbBroadEn_15980
- DNA Barcode:
- CTTAGCTTAGGGGTGTAGGCTCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC28B (79140)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480855
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79140 | CCDC28B | coiled-coil domain containi... | NM_024296.4 | 100% | 100% | |
| 2 | human | 79140 | CCDC28B | coiled-coil domain containi... | XM_017002307.1 | 100% | 100% | |
| 3 | human | 79140 | CCDC28B | coiled-coil domain containi... | XM_017002306.2 | 81.3% | 79.8% | (many diffs) |
| 4 | human | 79140 | CCDC28B | coiled-coil domain containi... | NM_001301011.2 | 79% | 76.8% | (many diffs) |
| 5 | human | 79140 | CCDC28B | coiled-coil domain containi... | XM_011542113.3 | 79% | 76.8% | (many diffs) |
| 6 | human | 79140 | CCDC28B | coiled-coil domain containi... | XM_017002308.2 | 56.8% | 55% | (many diffs) |
| 7 | human | 79140 | CCDC28B | coiled-coil domain containi... | XM_017002309.2 | 56.8% | 55% | (many diffs) |
| 8 | mouse | 66264 | Ccdc28b | coiled coil domain containi... | NM_025455.2 | 88.8% | 96.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 666
- ORF length:
- 600
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgacaaaaag aagaaacgga gtcccaagcc ctgcctggcc cagccagccc 121 aggccccagg cacactacgg agggtccctg tgcctaccag ccacagcggc tccttggccc 181 taggacttcc tcatctgcca tcccccaagc agcgggccaa gttcaagaga gtaggcaaag 241 agaagtgccg cccagtcctg gctggaggtg gaagcggctc tgcaggcacg cccctgcagc 301 actccTTCCT GACCGAGGTG ACTGATGTCT ATGAGATGGA GGGGGGACTC CTGAACCTGC 361 TCAATGATTT CCACTCTGGC CGGCTGCAGG CCTTCGGGAA GGAATGCTCC TTTGAGCAGC 421 TGGAGCACGT TCGGGAGATG CAGGAGAAGC TAGCCCGGCT GCACTTCAGC CTGGATGTGT 481 GTGGGGAGGA GGAGGACGAT GAAGAGGAAG AGGATGGGGT CACTGAGGGG CTGCCAGAGG 541 AGCAGAAGAA GACAATGGCT GACCGTAACC TGGACCAGCT GCTTAGCAAT CTGGAAGACC 601 TCAGTAATTC TATCCAGAAG CTGCACCTGG CCGAGAACGC CGAGCCTGAG GAGCAGTCCG 661 CTGCGTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 721 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 781 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACTTAGCTTA GGGGTGTAGG CTCCTACGCG 841 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt