Transcript: Human XM_017002309.2

PREDICTED: Homo sapiens coiled-coil domain containing 28B (CCDC28B), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CCDC28B (79140)
Length:
1653
CDS:
1106..1453

Additional Resources:

NCBI RefSeq record:
XM_017002309.2
NBCI Gene record:
CCDC28B (79140)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017002309.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000167892 CCTGAACCTGCTCAATGATTT pLKO.1 1390 CDS 100% 13.200 9.240 N CCDC28B n/a
2 TRCN0000167920 CAAGTTCAAGAGAGTAGGCAA pLKO.1 1258 CDS 100% 2.640 1.848 N CCDC28B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017002309.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15980 pDONR223 0% 56.8% 55% None (many diffs) n/a
2 ccsbBroad304_15980 pLX_304 0% 56.8% 55% V5 (many diffs) n/a
3 TRCN0000480855 CTTAGCTTAGGGGTGTAGGCTCCT pLX_317 59.8% 56.8% 55% V5 (many diffs) n/a
4 ccsbBroadEn_12548 pDONR223 100% 47.1% 45.6% None (many diffs) n/a
5 ccsbBroad304_12548 pLX_304 0% 47.1% 45.6% V5 (many diffs) n/a
6 TRCN0000470129 GTTGGCCATCACCTACGCAATTAC pLX_317 44% 47.1% 45.6% V5 (many diffs) n/a
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