Construct: ORF TRCN0000480863
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003488.2_s317c1
- Derived from:
- ccsbBroadEn_14555
- DNA Barcode:
- TCACCCTAAAATTATGTTTGATGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BUB1 (699)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480863
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 699 | BUB1 | BUB1 mitotic checkpoint ser... | NM_004336.5 | 100% | 100% | |
| 2 | human | 699 | BUB1 | BUB1 mitotic checkpoint ser... | NM_001278616.1 | 98.1% | 98.1% | 26_27ins60 |
| 3 | human | 699 | BUB1 | BUB1 mitotic checkpoint ser... | NM_001278617.1 | 94.7% | 94.7% | 2625_2626ins171 |
| 4 | human | 699 | BUB1 | BUB1 mitotic checkpoint ser... | XR_923001.3 | 93% | 1_68del;2530_2570del;3365_3499del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3321
- ORF length:
- 3255
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga caccccggaa aatgtccttc agatgcttga agcccacatg cagagctaca 121 agggcaatga ccctcttggt gaatgggaaa gatacataca gtgggtagaa gagaattttc 181 ctgagaataa agaatacttg ataactttac tagaacattt aatgaaggaa tttttagata 241 agaagaaata ccacaatgac ccaagattca tcagttattg tttaaaattt gctgagtaca 301 acagtgacct ccatcaattt tttgagtttc tgtacaacca tgggattgga accctgtcat 361 cccctctgta cattgcctgg gcggggcatc tggaagccca aggagagctg cagcatgcca 421 gtgctgtcct tcagagagga attcaaaacc aggctgaacc cagagagttc ctgcaacaac 481 aatacaggtt atttcagaca cgcctcactg aaacccattt gccagctcaa gctagaacct 541 cagaacctct gcataatgtt caggttttaa atcaaatgat aacatcaaaa tcaaatccag 601 gaaataacat ggcctgcatt tctaagaatc agggttcaga gctttctgga gtgatatctt 661 cagcttgtga taaagagtca aatatggaac gaagagtgat cacgatttct aaatcagaat 721 attctgtgca ctcatctttg gcatccaaag ttgatgttga gcaggttgtt atgtattgca 781 aggagaagct tattcgtggg gaatcagaat tttcctttga agaattgaga gcccagaaat 841 acaatcaacg gagaaagcat gagcaatggg taaatgaaga cagacattat atgaaaagga 901 aagaagcaaa tgcttttgaa gaacagctat taaaacagaa aatggatgaa cttcataaga 961 agttgcatca ggtggtggag acatcccatg aggatctgcc cgcttcccag gaaaggtccg 1021 aggttaatcc agcacgtatg gggccaagtg taggctccca gcaggaactg agagcgccat 1081 gtcttccagt aacctatcag cagacaccag tgaacatgga aaagaaccca agagaggcac 1141 ctcctgttgt tcctcctttg gcaaatgcta tttctgcagc tttggtgtcc ccagccacca 1201 gccagagcat tgctcctcct gttcctttga aagcccagac agtaacagac tccatgtttg 1261 cagtggccag caaagatgct ggatgtgtga ataagagtac tcatgaattc aagccacaga 1321 gtggagcaga gatcaaagaa gggtgtgaaa cacataaggt tgccaacaca agttcttttc 1381 acacaactcc aaacacatca ctgggaatgg ttcaggcaac gccatccaaa gtgcagccat 1441 cacccaccgt gcacacaaaa gaagcattag gtttcatcat gaatatgttt caggctccta 1501 cacttcctga tatttctgat gacaaagatg aatggcaatc tctagatcaa aatgaagatg 1561 catttgaagc ccagtttcaa aaaaatgtaa ggtcatctgg ggcttgggga gtcaataaga 1621 tcatctcttc tttgtcatct gcttttcatg tgtttgaaga tggaaacaaa gaaaattatg 1681 gattaccaca gcctaaaaat aaacccacag gagccaggac ctttggagaa cgctctgtca 1741 gcagacttcc ttcaaaacca aaggaggaag tgcctcatgc tgaagagttt ttggatgact 1801 caactgtatg gggtattcgc tgcaacaaaa ccctggcacc cagtcctaag agcccaggag 1861 acttcacatc tgctgcacaa cttgcgtcta caccattcca caagcttcca gtggagtcag 1921 tgcacatttt agaagataaa gaaaatgtgg tagcaaaaca gtgtacccag gcgactttgg 1981 attcttgtga ggaaaacatg gtggtgcctt caagggatgg aaaattcagt ccaattcaag 2041 agaaaagccc aaaacaggcc ttgtcgtctc acatgtattc agcatcctta cttcgtctga 2101 gccagcctgc tgcaggtggg gtacttacct gtgaggcaga gttgggcgtt gaggcttgca 2161 gactcacaga cactgacgct gccattgcag aagatccacc agatgctatt gctgggctcc 2221 aagcagaatg gatgcagatg agttcacttg ggactgttga tgctccaaac ttcattgttg 2281 ggaacccatg ggatgataag ctgattttca aacttttatc tgggctttct aaaccagtga 2341 gttcctatcc aaatactttt gaatggcaat gtaaacttcc agccatcaag cccaagactg 2401 aatttcaatt gggttctaag ctggtctatg tccatcacct tcttggagaa ggagcctttg 2461 cccaggtgta cgaagctacc cagggagatc tgaatgatgc taaaaataaa cagaaatttg 2521 ttttaaaggt ccaaaagcct gccaacccct gggaattcta cattgggacc cagttgatgg 2581 aaagactaaa gccatctatg cagcacatgt ttatgaagtt ctattctgcc cacttattcc 2641 agaatggcag tgtattagta ggagagctct acagctatgg aacattatta aatgccatta 2701 acctctataa aaatacccct gaaaaagtga tgcctcaagg tcttgtcatc tcttttgcta 2761 tgagaatgct ttacatgatt gagcaagtgc atgactgtga aatcattcat ggagacatta 2821 aaccagacaa tttcatactt ggaaacggat ttttggaaca ggatgatgaa gatgatttat 2881 ctgctggctt ggcactgatt gacctgggTC AGAGTATAGA TATGAAACTT TTTCCAAAAG 2941 GAACTATATT CACAGCAAAG TGTGAAACAT CTGGTTTTCA GTGTGTTGAG ATGCTCAGCA 3001 ACAAACCATG GAACTACCAG ATCGATTACT TTGGGGTTGC TGCAACAGTA TATTGCATGC 3061 TCTTTGGCAC TTACATGAAA GTGAAAAATG AAGGAGGAGA GTGTAAGCCT GAAGGTCTTT 3121 TTAGAAGGCT TCCTCATTTG GATATGTGGA ATGAATTTTT TCATGTTATG TTGAATATTC 3181 CAGATTGTCA TCATCTTCCA TCTTTGGATT TGTTAAGGCA AAAGCTGAAG AAAGTATTTC 3241 AACAACACTA TACTAACAAG ATTAGGGCCC TACGTAATAG GCTAATTGTA CTGCTCTTAG 3301 AATGTAAGCG TTCACGAAAA TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 3361 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 3421 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATCAC CCTAAAATTA 3481 TGTTTGATGA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt