Construct: ORF TRCN0000480867
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002269.1_s317c1
- Derived from:
- ccsbBroadEn_14256
- DNA Barcode:
- ATGGGCCCGATCGCCAATGAGTTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- METTL16 (79066)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480867
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79066 | METTL16 | methyltransferase like 16 | NM_024086.4 | 39.2% | (many diffs) | |
| 2 | mouse | 67493 | Mettl16 | methyltransferase like 16 | NM_026197.3 | 36.7% | (many diffs) | |
| 3 | mouse | 67493 | Mettl16 | methyltransferase like 16 | XM_006533998.3 | 36.7% | (many diffs) | |
| 4 | mouse | 67493 | Mettl16 | methyltransferase like 16 | XM_006533999.2 | 36.7% | (many diffs) | |
| 5 | mouse | 67493 | Mettl16 | methyltransferase like 16 | XM_006534000.3 | 28.9% | (many diffs) | |
| 6 | mouse | 67493 | Mettl16 | methyltransferase like 16 | XM_017314719.1 | 28.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 72
- ORF length:
- 6
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctgagtaaat caatgcatgc aagaaataga tacaaggaca aacctcctga 121 ctttgcatat ctggcatcca aatatccaga ttttaagcag catgttcaga taaatctgaa 181 tggaagagtg agccttaatt ttaaagaccc cgaagcagtc agagctctga cgtgtactct 241 cctaagggaa gattttggac tttctattga tattccattg gagagactaa ttcccacagt 301 tccctTGAGA CTCAACTATA TTCACTGGGT AGAAGATCTG ATCGGTCACC AGGATTCTGA 361 CAAAAGTACT CTCCGAAGAG GAATTGACAT AGGCACGGGG GCATCTTGCA TCTACCCCTT 421 ACTTGGAGCA ACCTTGAATG GCTGGTATTT CCTCGCAACA GAAGTGGATG ATATGTGTTT 481 CAACTATGCA AAGAAAAATG TGGAACAGAA TAACTTATCT GATCTCATAA AAGTGGTGAA 541 AGTGCCACAG AAGACACTCC TGATGGATGC TCTTAAAGAA GAATCTGAGA TAATCTATGA 601 CTTTTGCATG TGCAACCCTC CCTTTTTTGC CAATCAATTG GAAGCCAAGG GAGTAAACTC 661 ACGAAATCCT CGAAGACCTC CGCCTAGTTC TGTTAATACA GGAGACTCGC TTGAACCTGG 721 GAGGTGGAGG TTGCAGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT 781 CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG 841 TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAATGGGCCC GATCGCCAAT 901 GAGTTTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt