Construct: ORF TRCN0000480873
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017133.1_s317c1
- Derived from:
- ccsbBroadEn_10814
- DNA Barcode:
- GTAATCCGAGGTCCCTTCATGGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EYA2 (2139)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480873
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2139 | EYA2 | EYA transcriptional coactiv... | NM_005244.5 | 95.4% | 95.3% | 294_365del;1082G>C |
| 2 | human | 2139 | EYA2 | EYA transcriptional coactiv... | XM_017027721.2 | 89.9% | 89.7% | 294_365del;886_887ins90;992G>C |
| 3 | human | 2139 | EYA2 | EYA transcriptional coactiv... | XM_005260327.2 | 86.6% | 80.1% | (many diffs) |
| 4 | human | 2139 | EYA2 | EYA transcriptional coactiv... | NM_172110.3 | 80.7% | 80.6% | 294_365del;1082G>C;1197_1198ins237 |
| 5 | mouse | 14049 | Eya2 | EYA transcriptional coactiv... | NM_001271962.1 | 79.1% | 83.8% | (many diffs) |
| 6 | mouse | 14049 | Eya2 | EYA transcriptional coactiv... | NM_001271963.1 | 79.1% | 83.8% | (many diffs) |
| 7 | mouse | 14049 | Eya2 | EYA transcriptional coactiv... | NM_010165.3 | 79.1% | 83.8% | (many diffs) |
| 8 | mouse | 14049 | Eya2 | EYA transcriptional coactiv... | XM_006498739.3 | 71.3% | 77.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1608
- ORF length:
- 1542
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt agaactagtg atctcaccca gcctcactgt aaacagcgat tgtctggata 121 aactgaagtt taaccgtgct gacgctgctg tgtggactct gagtgacaga caaggcatca 181 ccaaatcggc ccccctgaga gtgtcccagc tcttctccag atcttgccca cgtgtcctcc 241 cccgccagcc ttccacagcc atggcagcct acggccagac gcagtacagt gcggggatcc 301 agcaggctac cccctataca gcttacccac ctccagcaca agcctatgga atcccttcct 361 tcagcacctc acccactgga cagagcccat acacctacca gatgcacggc acaacagggt 421 tctatcaagg aggaaatgga ctgggcaacg cagccggttt cgggagtgtg caccaggact 481 atccttccta ccccggcttc ccccagagcc agtaccccca gtattacggc tcatcctaca 541 accctcccta cgtcccggcc agcagcatct gcccttcgcc cctctccacg tccacctacg 601 tcctccagga ggcatctcac aacgtcccca accagagttc cgagtcactt gctggtgaat 661 acaacacaca caatggacct tccacaccag cgaaagaggg agacacagac aggccgcacc 721 gggcctccga cgggaagctc cgaggccggt ctaagaggag cagtgacccg tccccggcag 781 gggacaatga gattgagcgt gtgttcgtgt gggacttgga tgagacaata attatttttc 841 actccttact cacggggaca tttgcatcca gatacgggaa ggacaccacg acgtccgtgc 901 gcattggcct tatgatggaa gagatgatct tcaaccttgc agatacacat ctgttcttca 961 atgacctgga ggattgtgac cagatccacg ttgatgacgt ctcatcagat gacaatggcc 1021 aagatttaag cacatacaac ttctccgctg acggcttcca cagttcggcc ccagcagcca 1081 acctgtgcct gggctctggc gtgcacggcg gcgtggactg gatgaggaag ctggccttcc 1141 gctaccggcg ggtgaaggag atgtacaata cctacaagaa caacgttggt gggttgatag 1201 gcactcccaa aagggagacc tggctacagc TCCGAGCTGA GCTGGAAGCT CTCACAGACC 1261 TCTGGCTGAC CCACTCCCTG AAGGCACTAA ACCTCATCAA CTCCCGGCCC AACTGTGTCA 1321 ATGTGCTGGT CACCACCACT CAACTAATTC CTGCCCTGGC CAAAGTCCTG CTATATGGCC 1381 TGGGGTCTGT GTTTCCTATT GAGAACATCT ACAGTGCAAC CAAGACAGGG AAGGAGAGCT 1441 GCTTCGAGAG GATAATGCAG AGATTCGGCA GAAAAGCTGT CTACGTGGTG ATCGGTGATG 1501 GTGTGGAAGA GGAGCAAGGA GCGAAAAAGC ACAACATGCC TTTCTGGCGG ATATCCTGCC 1561 ACGCAGACCT GGAGGCACTG AGGCACGCCC TGGAGCTGGA GTATTTATAC CCAACTTTCT 1621 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1681 CGTAGTAATG AACTAGCCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1741 GTGGAAAGGA CGAGTAATCC GAGGTCCCTT CATGGCAACG CGTTAAGTCg acaatcaacc 1801 tctggattac aaaatttgtg aaagatt