Construct: ORF TRCN0000480873

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017133.1_s317c1
Derived from:
ccsbBroadEn_10814
DNA Barcode:
GTAATCCGAGGTCCCTTCATGGCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EYA2 (2139)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480873
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2139 EYA2 EYA transcriptional coactiv... NM_005244.5 95.4% 95.3% 294_365del;1082G>C
2 human 2139 EYA2 EYA transcriptional coactiv... XM_017027721.2 89.9% 89.7% 294_365del;886_887ins90;992G>C
3 human 2139 EYA2 EYA transcriptional coactiv... XM_005260327.2 86.6% 80.1% (many diffs)
4 human 2139 EYA2 EYA transcriptional coactiv... NM_172110.3 80.7% 80.6% 294_365del;1082G>C;1197_1198ins237
5 mouse 14049 Eya2 EYA transcriptional coactiv... NM_001271962.1 79.1% 83.8% (many diffs)
6 mouse 14049 Eya2 EYA transcriptional coactiv... NM_001271963.1 79.1% 83.8% (many diffs)
7 mouse 14049 Eya2 EYA transcriptional coactiv... NM_010165.3 79.1% 83.8% (many diffs)
8 mouse 14049 Eya2 EYA transcriptional coactiv... XM_006498739.3 71.3% 77.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1608
ORF length:
1542
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt agaactagtg atctcaccca gcctcactgt aaacagcgat tgtctggata
121aactgaagtt taaccgtgct gacgctgctg tgtggactct gagtgacaga caaggcatca
181ccaaatcggc ccccctgaga gtgtcccagc tcttctccag atcttgccca cgtgtcctcc
241cccgccagcc ttccacagcc atggcagcct acggccagac gcagtacagt gcggggatcc
301agcaggctac cccctataca gcttacccac ctccagcaca agcctatgga atcccttcct
361tcagcacctc acccactgga cagagcccat acacctacca gatgcacggc acaacagggt
421tctatcaagg aggaaatgga ctgggcaacg cagccggttt cgggagtgtg caccaggact
481atccttccta ccccggcttc ccccagagcc agtaccccca gtattacggc tcatcctaca
541accctcccta cgtcccggcc agcagcatct gcccttcgcc cctctccacg tccacctacg
601tcctccagga ggcatctcac aacgtcccca accagagttc cgagtcactt gctggtgaat
661acaacacaca caatggacct tccacaccag cgaaagaggg agacacagac aggccgcacc
721gggcctccga cgggaagctc cgaggccggt ctaagaggag cagtgacccg tccccggcag
781gggacaatga gattgagcgt gtgttcgtgt gggacttgga tgagacaata attatttttc
841actccttact cacggggaca tttgcatcca gatacgggaa ggacaccacg acgtccgtgc
901gcattggcct tatgatggaa gagatgatct tcaaccttgc agatacacat ctgttcttca
961atgacctgga ggattgtgac cagatccacg ttgatgacgt ctcatcagat gacaatggcc
1021aagatttaag cacatacaac ttctccgctg acggcttcca cagttcggcc ccagcagcca
1081acctgtgcct gggctctggc gtgcacggcg gcgtggactg gatgaggaag ctggccttcc
1141gctaccggcg ggtgaaggag atgtacaata cctacaagaa caacgttggt gggttgatag
1201gcactcccaa aagggagacc tggctacagc TCCGAGCTGA GCTGGAAGCT CTCACAGACC
1261TCTGGCTGAC CCACTCCCTG AAGGCACTAA ACCTCATCAA CTCCCGGCCC AACTGTGTCA
1321ATGTGCTGGT CACCACCACT CAACTAATTC CTGCCCTGGC CAAAGTCCTG CTATATGGCC
1381TGGGGTCTGT GTTTCCTATT GAGAACATCT ACAGTGCAAC CAAGACAGGG AAGGAGAGCT
1441GCTTCGAGAG GATAATGCAG AGATTCGGCA GAAAAGCTGT CTACGTGGTG ATCGGTGATG
1501GTGTGGAAGA GGAGCAAGGA GCGAAAAAGC ACAACATGCC TTTCTGGCGG ATATCCTGCC
1561ACGCAGACCT GGAGGCACTG AGGCACGCCC TGGAGCTGGA GTATTTATAC CCAACTTTCT
1621TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
1681CGTAGTAATG AACTAGCCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
1741GTGGAAAGGA CGAGTAATCC GAGGTCCCTT CATGGCAACG CGTTAAGTCg acaatcaacc
1801tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)

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