Construct: ORF TRCN0000480876
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015787.1_s317c1
- Derived from:
- ccsbBroadEn_08764
- DNA Barcode:
- ACCTTGGAAACCCCCCCGGGTTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MEAK7 (57707)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480876
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57707 | MEAK7 | MTOR associated protein, ea... | NM_020947.4 | 99.7% | 99.3% | 658A>G;799T>C;1342G>T |
| 2 | human | 57707 | MEAK7 | MTOR associated protein, ea... | XM_005256075.2 | 99.7% | 99.3% | 658A>G;799T>C;1342G>T |
| 3 | human | 57707 | MEAK7 | MTOR associated protein, ea... | XM_006721240.2 | 99.7% | 99.3% | 658A>G;799T>C;1342G>T |
| 4 | human | 57707 | MEAK7 | MTOR associated protein, ea... | XM_011523248.2 | 99.7% | 99.3% | 658A>G;799T>C;1342G>T |
| 5 | human | 57707 | MEAK7 | MTOR associated protein, ea... | XM_011523249.2 | 99.7% | 99.3% | 658A>G;799T>C;1342G>T |
| 6 | human | 57707 | MEAK7 | MTOR associated protein, ea... | XM_017023510.1 | 99.7% | 99.3% | 658A>G;799T>C;1342G>T |
| 7 | human | 57707 | MEAK7 | MTOR associated protein, ea... | XM_017023511.1 | 99.7% | 99.3% | 658A>G;799T>C;1342G>T |
| 8 | human | 57707 | MEAK7 | MTOR associated protein, ea... | XM_005256074.3 | 92.1% | 91.7% | (many diffs) |
| 9 | human | 57707 | MEAK7 | MTOR associated protein, ea... | XM_017023512.1 | 91.7% | 89% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1434
- ORF length:
- 1368
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gaacagcaga agccgtgtgg ggcggagctt ttgttcacag tttcttcctg 121 aggaacaggc agagattgat caattgtttg atgctctgtc atcagataaa aacagcccga 181 atgtctcatc caaatccttc tctctgaagg cactacagaa ccacgtcggg gaagctcttc 241 ccccagagat ggtcaccagg ctgtatgatg gcatgcggag ggtcgacctg acagggaagg 301 cgaagggacc cagtgagaac gtgtcccagg agcagttcac agcatccatg tcccacctgt 361 tgaaaggaaa ctccgaggag aagagtctca tgattatgaa aatgatttct gccacagaag 421 gtcccgtgaa ggccagagaa gtccaaaagt ttacagagga tctggttggc tctgtggtgc 481 acgtgctaag ccacagacag gagctgagag gctggactgg gaaggaagcc ccagggccca 541 acccccgggt gcaggtgctg gctgctcagc tgctctctga catgaagctg caagatggca 601 agagacttct ggggccccag tggctggact atgactgtga ccgagctgtg atcgaggact 661 gggtgttcag ggtcccccat gtggccatat tcctgagtgt ggtcatttgc aagggctttc 721 tcgtcctgtg ctcgtctctt gatctgacta ccctggtccc tgagcgtcaa gtggaccagg 781 gcaggggttt tgagagcatc ctggatgtcc tctctgtcat gtacatcaac gcccagctgc 841 ctcgggagca gcggcaccgc tggcgcctgc tcttttcgtc tgagctccat ggacacagct 901 tctcccagct ctgtggccac atcactcacc ggggaccctg tgtggctgtc ctcgaggacc 961 atgacaagca tgtgttcggt gggtttgcct cttgctcttg ggaggtgaag ccTCAGTTTC 1021 AAGGGGACAA CAGATGCTTC CTGTTCTCCA TCTGCCCCAG CATGGCTGTG TACACACACA 1081 CGGGCTACAA CGACCACTAC ATGTACTTGA ACCATGGACA GCAGACGATC CCGAACGGAC 1141 TGGGTATGGG GGGGCAGCAC AATTACTTTG GGCTTTGGGT GGATGTTGAT TTTGGGAAAG 1201 GACACAGCAG AGCCAAGCCC ACGTGCACCA CGTACAACAG CCCGCAGCTG TCGGCTCAGG 1261 AGAACTTCCA GTTTGATAAG ATGGAGGTGT GGGCGGTTGG AGACCCCTCA GAGGAGCAGT 1321 TGGCCAAGGG CAACAAGAGC ATCCTGGATG CGGACCCTGA GGCCCAGGCC CTGCTGGAGA 1381 TCAGTGGGCA TTCGCGCCAC AGCGAATGGC TCCGGGAAGT CCCGGACGAT GAATGCCCAA 1441 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1501 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1561 TATCTTGTGG AAAGGACGAA CCTTGGAAAC CCCCCCGGGT TGCACGCGTT AAGTCgacaa 1621 tcaacctctg gattacaaaa tttgtgaaag att