Construct: ORF TRCN0000480884
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012662.1_s317c1
- Derived from:
- ccsbBroadEn_15712
- DNA Barcode:
- TCGGCTATGGGGTGTTTAACATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPNMB (10457)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480884
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10457 | GPNMB | glycoprotein nmb | XM_017011678.2 | 38.7% | 35.2% | (many diffs) |
| 2 | human | 10457 | GPNMB | glycoprotein nmb | XM_005249578.3 | 37.8% | 34.4% | (many diffs) |
| 3 | human | 10457 | GPNMB | glycoprotein nmb | XM_017011677.2 | 35.9% | 32.7% | (many diffs) |
| 4 | human | 10457 | GPNMB | glycoprotein nmb | NM_002510.3 | 35.8% | 32.6% | (many diffs) |
| 5 | human | 10457 | GPNMB | glycoprotein nmb | NM_001005340.2 | 35.1% | 32% | (many diffs) |
| 6 | human | 10457 | GPNMB | glycoprotein nmb | XM_017011676.2 | 33.5% | 30.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 684
- ORF length:
- 618
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga atgtctctac tatttcctgg gatttctgct cctggctgca agattgccac 121 ttgatgccgc caaacgattt catgatgtgc tgggcaatga aagaccttct gcttacatga 181 gggagcacaa tcaattaaat ggctggTCTT CTGATGAAAA TGACTGGAAT GAAAAACTCT 241 ACCCAGTGTG GAAGCGGGGA GACATGAGGT GGAAAAACTC CTGGAAGGGA GGCCGTGTGC 301 AGGCGGTCCT GACCAGTGAC TCACCAGCCC TCGTGGGCTC AAATATAACA TTTGCGGTGA 361 ACCTGATATT CCCTAGATGC CAAAAGGAAG ATGCCAATGG CAACATAGTC TATGAGAAGA 421 ACTGCAGAAA TGAGGCTGGT TTATCTGCTG ATCCATATGT TTACAACTGG ACAGCATGGT 481 CAGAGGACAG TGACGGGGAA AATGGCACCG GCCAAAGCCA TCATAACGTC TTCCCTGATG 541 GGAAACCTTT TCCTCACCAC CCCGGATGGA GAAGATGGAA TTTCATCTAC GTCTTCCACA 601 CACTTGGTTG GCTTTTACAA ACCCCTAAGC TTCTTCTTTA CCTTTCCTTA AAATTTCAAC 661 CTTCTCTTTT CTTACTCTAT AATTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 721 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 781 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT CGGCTATGGG 841 GTGTTTAACA TCAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 901 att