Construct: ORF TRCN0000480888
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011608.1_s317c1
- Derived from:
- ccsbBroadEn_04172
- DNA Barcode:
- AGGCTGACATTGAAGACGACCGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MZT2B (80097)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480888
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80097 | MZT2B | mitotic spindle organizing ... | NM_025029.5 | 100% | 100% | |
| 2 | human | 653784 | MZT2A | mitotic spindle organizing ... | NM_001085365.2 | 97.8% | 96.2% | (many diffs) |
| 3 | human | 653784 | MZT2A | mitotic spindle organizing ... | XM_024453048.1 | 74% | 72.7% | (many diffs) |
| 4 | human | 653784 | MZT2A | mitotic spindle organizing ... | XM_024453049.1 | 74% | 72.7% | (many diffs) |
| 5 | human | 80097 | MZT2B | mitotic spindle organizing ... | NM_001330282.2 | 72.4% | 72.4% | 318_497del |
| 6 | human | 80097 | MZT2B | mitotic spindle organizing ... | NM_001330284.2 | 60.2% | 38.2% | 170_171ins149;326_390del |
| 7 | human | 653784 | MZT2A | mitotic spindle organizing ... | XM_005263742.3 | 60% | 52% | (many diffs) |
| 8 | human | 653784 | MZT2A | mitotic spindle organizing ... | XM_017004744.2 | 58.5% | 51.5% | (many diffs) |
| 9 | human | 653784 | MZT2A | mitotic spindle organizing ... | XM_024453050.1 | 58.5% | 51.5% | (many diffs) |
| 10 | human | 653784 | MZT2A | mitotic spindle organizing ... | XM_024453051.1 | 57.4% | 56.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 540
- ORF length:
- 474
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcgcagggc gtagggcctg ggccggggtc ggcggcgccc ccggggctgg 121 aggcggCCCG GCAGAAGCTG GCGCTGCGGC GGAAGAAGGT GCTGAGCACC GAGGAGATGG 181 AGCTGTACGA GCTGGCGCAG GCGGCGGGCG GCGCTATCGA CCCCGACGTG TTCAAGATCC 241 TGGTGGACCT GCTGAAGCTG AACGTGGCCC CCCTCGCCGT CTTCCAGATG CTCAAGTCCA 301 TGTGTGCCGG GCAGAGGCTA GCGAGCGAGC CCCAGGACCC TGCGGCCGTG TCTCTGCCCA 361 CGTCGAGCGT GCCCGAGACC CGAGGGAGAA ACAAAGGCAG CGCTGCCCTC GGGGGAGCAT 421 TGGCCCTGGC GGAACGCAGC AGCCGCGAAG GATCCAGCCA GAGGATGCCA CGCCAGCCCA 481 GCGCTACCAG GCTGCCCAAG GGGGGCGGGC CTGGGAAGAG CCCTACACGG GGCAGCACCT 541 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 601 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 661 TTTATATATC TGTGGAAAGG ACGAAGGCTG ACATTGAAGA CGACCGTAAC GCGTTAAGTC 721 gacaatcaac ctctggatta caaaatttgt gaaagatt