Construct: ORF TRCN0000480889
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011301.1_s317c1
- Derived from:
- ccsbBroadEn_08477
- DNA Barcode:
- AACTGTCGAGCAAGCCGTTACTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTPAP (55149)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480889
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55149 | MTPAP | mitochondrial poly(A) polym... | NM_018109.3 | 99.7% | 99.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1815
- ORF length:
- 1746
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggttccc ggcgtggggc tcttgacccg tttgaacctg tgtgcccgga 121 gaagaactcg agtccagcgg cctatcgtca ggcttttgag ttgcccagga actgtggcca 181 aagaccttag gagagacgag cagccttcag ggagcgtgga gacaggcttt gaagacaaga 241 ttcccaaaag gagattctct gagatgcaaa atgaaagacg agaacaggca cagcggactg 301 ttttaataca ttgcccagag aaaatcagtg aaaacaagtt tcttaaatat ttatcccaat 361 ttggacctat taataatcat ttcttctatg aaagctttgg tctctatgct gtcgtagaat 421 tttgccaaaa ggaaagcata ggttcactgc agaatgggac tcatactcca agcacggcca 481 tggagactgc aattccattc agatcacgtt tcttcaatct gaagttgaaa aaccagactt 541 ctgaacggtc acgcgtacgg tcaagtaatc agttgccacg ttcaaacaag cagctttttg 601 aattactttg ttatgcagaa agtatagacg atcagctgaa cactctcttg aaggagttcc 661 agctaacaga ggagaacact aagctccgat atctcacctg ttctcttatt gaagacatgg 721 ccgccgcgca ttttccagac tgcatagtca gaccctttgg ctcctcagtc aacacttttg 781 ggaagttagg atgtgatttg gacatgtttt tggatctaga tgaaaccaga aacctcagcg 841 ctcacaagat ctcaggaaat tttctgatgg aatttcaagt gaaaaatgtt ccttcagaaa 901 gaattgcaac tcagaagatc ctgtctgtgt taggagagtg ccttgaccac tttggccctg 961 gctgtgtggg tgtgcaaaaa atattaaatg cccggtgtcc gctcgtgagg ttctcacacc 1021 aggcctccgg atttcagtgt gatttgacta cgaacaatag gattgccttg acaagttccg 1081 aactccttta tatatatggt gccctagact caagagtgag agccttggtg ttcagtgtac 1141 ggtgctgggc tcgagcacat tcactaacaa gtagtattcc tggtgcatgg attacaaatt 1201 tctcccttac aatgatggtc atcttttttc tccagagaag atcaccccct attcttccaa 1261 cactagattc cttaaaaacc ctagcagatg cagaagataa atgtgtaata gaaggcaaca 1321 accgcacatt tgttcgtgac ttgagtagaa ttaaaccttc acagaacaca gaaacattag 1381 aattactact gaaggaattt tttgagtatt ttggcaattt tgctttcgat aaaaattcca 1441 taaatattcg acaggGAAGG GAGCAAAACA AACCTGATTC TTCTCCCCTG TACATTCAGA 1501 ATCCATTTGA AACTTCTCTC AACATAAGCA AAAATGTAAG TCAAAGCCAG CTGCAAAAAT 1561 TTGTAGATTT GGCCCGAGAA AGTGCCTGGA TTTTACAACA GGAAGATACA GATCGACCTT 1621 CCATATCAAG TAATCGGCCC TGGGGGCTGG TATCCCTATT GCTACCATCT GCTCCAAACA 1681 GAAAGTCCTT TACCAAGAAG AAAAACAATA AGTTTGCAAT TGAAACAGTC AAGAACTTGC 1741 TAGAATCTTT AAAAGGTAAC AGAACAGAAA ATTTCACAAA AACCAGTGGG AAGAGAACAA 1801 TTAGTACTCA GACATTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1861 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1921 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AACTGTCGAG CAAGCCGTTA 1981 CTCTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt