Construct: ORF TRCN0000480903
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006742.1_s317c1
- Derived from:
- ccsbBroadEn_03597
- DNA Barcode:
- ATGACACTATTGTTAGTTGACTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPRC5D (55507)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480903
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55507 | GPRC5D | G protein-coupled receptor ... | NM_018654.1 | 100% | 100% | |
| 2 | human | 55507 | GPRC5D | G protein-coupled receptor ... | XM_005253421.3 | 100% | 100% | |
| 3 | human | 55507 | GPRC5D | G protein-coupled receptor ... | XM_017019582.2 | 100% | 100% | |
| 4 | human | 55507 | GPRC5D | G protein-coupled receptor ... | XM_017019583.2 | 86.8% | 86.3% | 892_893insGAGCCC;898T>A;900_901ins129 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1104
- ORF length:
- 1035
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtacaaggac tgcatcgagt ccactggaga ctattttctt ctctgtgacg 121 ccgaggggcc atggggcatc attctggagt ccctggccat acttggcatc gtggtcacaa 181 ttctgctact cttagcattt ctcttcctca tgcgaaagat ccaagactgc agccagtgga 241 atgtcctccc cacccagctc ctcttcctcc tgagtgtcct ggggctcttc ggactcgctt 301 ttgccttcat catcgagctc aatcaacaaa ctgcccccgt acgctacttt ctctttgggg 361 ttctctttgc tctctgtttc tcatgcctct tagctcatgc ctccaatcta gtgaagctgg 421 ttcggggttg tgtctccttc tcctggacga caattctgtg cattgctatt ggttgcagtc 481 tgttgcaaat cattattgcc actgagtatg tgactctcat catgaccaga ggtatgatgt 541 ttgtgaatat gacaccctgc cagctcaatg tggactttgt tgtactcctg gtctatgtcc 601 tcttcctgat ggccctcaca ttcttcgtct ccaaagccac cttctgtggc ccgtgtgaga 661 actggaagca gcatggaagg ctcatctttA TCACTGTGCT CTTCTCCATC ATCATCTGGG 721 TGGTGTGGAT CTCCATGCTC CTGAGAGGCA ACCCGCAGTT CCAGCGACAG CCCCAGTGGG 781 ACGACCCGGT CGTCTGCATT GCTCTGGTCA CCAACGCATG GGTTTTCCTG CTGCTGTACA 841 TCGTCCCTGA GCTCTGCATT CTCTACAGAT CGTGTAGACA GGAGTGCCCT TTACAAGGCA 901 ATGCCTGCCC CGTCACAGCC TACCAACACA GCTTCCAAGT GGAGAACCAG GAGCTCTCCA 961 GAGCCCGAGA CAGTGATGGA GCTGAGGAGG ATGTAGCATT AACTTCATAT GGTACTCCCA 1021 TTCAGCCGCA GACTGTTGAT CCCACACAAG AGTGTTTCAT CCCACAGGCT AAACTAAGCC 1081 CCCAGCAAGA TGCAGGAGGA GTATTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1141 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1201 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA TGACACTATT 1261 GTTAGTTGAC TTAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1321 att