Construct: ORF TRCN0000480918

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000573.1_s317c1
Derived from:
ccsbBroadEn_04859
DNA Barcode:
CAACACGCTGGGCACGGTCGGCCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RFTN2 (130132)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480918
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 130132 RFTN2 raftlin family member 2 NM_144629.3 100% 100%
2 human 130132 RFTN2 raftlin family member 2 XM_011510597.3 99.8% 99.8% 1233_1234insAAG
3 human 130132 RFTN2 raftlin family member 2 XM_011510596.3 98.2% 98.2% 1232_1258del
4 human 130132 RFTN2 raftlin family member 2 XM_011510595.3 98% 98% 1234_1263del
5 human 130132 RFTN2 raftlin family member 2 XM_017003332.2 96.6% 96.6% 436_437ins51
6 human 130132 RFTN2 raftlin family member 2 XM_011510598.3 94.7% 94.7% 436_437ins51;1183_1212del
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1572
ORF length:
1503
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggggtgcgga cttagaaagc tagaagaccc tgatgatagc agccctggaa
121aaatattttc taccctgaag agaccgcaag tggaaacaaa gacagaattt gcttacgaat
181atgtattgct ggattttact ctacaagctt catcaaaccc agaagtcatc aagataaatt
241caattctgga tatagtaaca aaagtggaaa actattatct taaaggatat attgtcgggg
301ctattcatcc tgttatacaa cctgtggggc agcgaaaaca cctacctgca agttacctat
361acagagtggt gctattgcgc ttgaaattaa gcccaaagaa ttcggcagca cccagtggac
421aaagacgccc aaggctcgtg attgaggaat gtcccctaac ttctgaggca caaacaaatg
481acgcagcaaa agaactgata gaaaagatta atgtcgctgc taaaagagga atgaaatttg
541ttggattcat atcacagcat tattctccat ctaaattctg caatggaacc aaccatgatg
601gagatataga atcgatgcta catgtgagac acggttcaga tgaaaactgt agaagttgga
661atgaagggac gttaagtggg cagtcatctg aaagcggaat tgaggaagaa cttcatcatg
721aaagtggaca gtatcaaatg gaacaaaatg gcagccctac ttcctctaaa tcaagaaagg
781gagaagcctc agataacaaa ttgtatacag tcttcaatgc ttttgatgat gattcaacct
841cctgggccta tcaggaaggc atcctgtcaa tgaaagtaac aagaaaagga tcagtcatta
901gtacacttga tgctgattgg ctggagttaa ctacctttta ttataaacaa ggcttgtctt
961taattgattc ttttgtattc tgggaaacat ctaaagggga acatttgcct aaatctttgg
1021aaggattttt tatctatgaa gaagaaggtt ctggagttcc aggttctagt aggaaaggaa
1081atgatgccat cgtagtagaa caatggactg ttattgaggg atgtgaaatc aaaacagatt
1141atggcccTCT GCTGCACACT CTGGCTGAAT TCGGATGGCT TCTGACAAGC GTGTTGCCCA
1201CACCTGTATT GAGACATGAC AGTGAAGGGA ATTTGGCTAC AAAGCAGATC GTATTCCTTC
1261AGAGGCCAGT TATGTGGAAT TCAGCTGCTC AAACACCAGA TAAGAAAGCC AGCCGCCACA
1321TAAAAGGTGA AGACAAGAAT AAAGCCACTA GTAGAAGCAT CGGATTAGAC ACAACCTCGT
1381CACAACCTGC AGAGAGCAGA CACCTGCCTG AGGAGTGCCG CCTTTCTCCC TCCCGGGAAT
1441GCTGGACAAA GGAGGGAAGG CTGGCACAGC ACAACAGCTT CTCTGGGTTC AGCAGCAGTG
1501ACAACGTCCT CCGGGAATTA GACGACGGAC AGTTTGATCA GGAAGATGGA GTGACTCAGG
1561TCACTTGTAT GTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
1621ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
1681CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACAA CACGCTGGGC ACGGTCGGCC
1741AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)