Construct: ORF TRCN0000480938
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009071.1_s317c1
- Derived from:
- ccsbBroadEn_06432
- DNA Barcode:
- TCCGGGCTATGGCAATGTTAGACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IGFBP1 (3484)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480938
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3484 | IGFBP1 | insulin like growth factor ... | NM_000596.4 | 99.8% | 99.6% | 452T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 843
- ORF length:
- 777
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc agaggtcccc gttgctcgcg tctggctggt actgctcctg ctgactgtcc 121 aggtcggcgt gacagccggc gctccgtggc agtgcgcgcc ctgctccgcc gagaagctcg 181 cgctctgccc gccggtgtcc gcctcgtgct cggaggtcac ccggtccgcc ggctgcggct 241 gttgcccgat gtgcgccctg cctctgggcg ccgcgtgcgg cgtggcgact gcacgctgcg 301 cccggggact cagttgccgc gcgctgccgg gggagcagca acctctgcac gccctcaccc 361 gcggccaagg cgcctgcgtg caggagtctg acgccTCCGC TCCCCATGCT GCAGAGGCAG 421 GGAGCCCTGA AAGCCCAGAG AGCACGGAGA TAACTGAGGA GGAGCTCCTG GATAATTTCC 481 ATCTGATGGC CCCTTCTGAA GAGGATCATT CCATCCCTTG GGACGCCATC AGTACCTATG 541 ATGGCTCGAA GGCTCTCCAT GTCACCAACA TCAAAAAATG GAAGGAGCCC TGCCGAATAG 601 AACTCTACAG AGTCGTAGAG AGTTTAGCCA AGGCACAGGA GACATCAGGA GAAGAAATTT 661 CCAAATTTTA CCTGCCAAAC TGCAACAAGA ATGGATTTTA TCACAGCAGA CAGTGTGAGA 721 CATCCATGGA TGGAGAGGCG GGACTCTGCT GGTGCGTCTA CCCTTGGAAT GGGAAGAGGA 781 TCCCTGGGTC TCCAGAGATC AGGGGAGACC CCAACTGCCA GATATATTTT AATGTACAAA 841 ACTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 901 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 961 GGCTTTATAT ATCTTGTGGA AAGGACGATC CGGGCTATGG CAATGTTAGA CAACGCGTTA 1021 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt