Construct: ORF TRCN0000480942

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006084.3_s317c1
Derived from:
ccsbBroadEn_03756
DNA Barcode:
ACTAGACGAACTCCGTGGCATTGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PAK6 (56924)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480942
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 106821730 BUB1B-PAK6 BUB1B-PAK6 readthrough NM_001128628.2 100% 100%
2 human 106821730 BUB1B-PAK6 BUB1B-PAK6 readthrough NM_001128629.3 100% 100%
3 human 56924 PAK6 p21 (RAC1) activated kinase 6 NM_001276717.1 100% 100%
4 human 56924 PAK6 p21 (RAC1) activated kinase 6 NM_020168.5 100% 100%
5 human 56924 PAK6 p21 (RAC1) activated kinase 6 NM_001276718.2 93.3% 93.3% 1741_1742ins135
6 mouse 214230 Pak6 p21 protein (Cdc42/Rac)-act... NM_001033254.3 88.7% 92.5% (many diffs)
7 mouse 214230 Pak6 p21 protein (Cdc42/Rac)-act... NM_001145854.1 88.7% 92.5% (many diffs)
8 mouse 214230 Pak6 p21 protein (Cdc42/Rac)-act... XM_011239419.1 88.7% 92.5% (many diffs)
9 mouse 214230 Pak6 p21 protein (Cdc42/Rac)-act... XM_011239420.2 88.7% 92.5% (many diffs)
10 mouse 214230 Pak6 p21 protein (Cdc42/Rac)-act... XR_866265.2 51.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2109
ORF length:
2043
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtt ccgcaagaaa aagaagaaac gccctgagat ctcagcgcca cagaacttcc
121agcaccgtgt ccacacctcc ttcgacccca aagaaggcaa gtttgtgggc ctccccccac
181aatggcagaa catcctggac acactgcggc gccccaagcc cgtggtggac ccttcgcgaa
241tcacacgggt gcagctccag cccatgaaga cagtggtgcg gggcagcgcg atgcctgtgg
301atggctacat ctcggggctg ctcaacgaca tccagaagtt gtcagtcatc agctccaaca
361ccctgcgtgg ccgcagcccc accagccggc ggcgggcaca gtccctgggg ctgctggggg
421atgagcactg ggccaccgac ccagacatgt acctccagag cccccagtct gagcgcactg
481acccccacgg cctctacctc agctgcaacg ggggcacacc agcaggccac aagcagatgc
541cgtggcccga gccacagagc ccacgggtcc tgcccaatgg gctggctgca aaggcacagt
601ccctgggccc cgccgagttt cagggtgcct cgcagcgctg tctgcagctg ggtgcctgcc
661tgcagagctc cccaccagga gcctcgcccc ccacgggcac caataggcat ggaatgaagg
721ctgccaagca tggctctgag gaggcccggc cacagtcctg cctggtgggc tcagccacag
781gcaggccagg tggggaaggc agccctagcc ctaagacccg ggagagcagc ctgaagcgca
841ggctattccg aagcatgttc ctgtccactg ctgccacagc ccctccaagc agcagcaagc
901caggccctcc accacagagc aagcccaact cctctttccg accgccgcag aaagacaacc
961ccccaagcct ggtggccaag gcccagtcct tgccctcgga ccagccggtg gggaccttca
1021gccctctgac cacttcggat accagcagcc cccagaagtc cctccgcaca gccccggcca
1081caggccagct tccaggccgg tcttccccag cgggatcccc ccgcacctgg cacgcccaga
1141tcagcaccag caacctgtac ctgccccagg accccacggt tgccaagggt gccctggctg
1201gtgaggacac aggtgttgtg acacatgagc agttcaaggc tgcgctcagg atggtggtgg
1261accagggtga cccccggctg ctgctggaca gctacgtgaa gattggcgag ggctccaccg
1321gcatcgtctg cttggcccgg gagaagcact cgggccgcca ggtggccgtc aagatgatgg
1381acctcaggaa gcagcagcgc agggagctgc tcttcaacga ggtggtgatc atgcgggact
1441accagcactt caacgtggtg gagatgtaca agagctacct ggtgggcgag gagctgtggg
1501tgctcatgga gttcctgcag ggaggagccc tcacagacat cgtctcccaa gtcaggctga
1561atgaggagca gattgccact gtgtgtgagg ctgtgctgca ggccctggcc tacctgcatg
1621ctcagggtgt catccaccgg gacatcaaga gtgactccat cctgctgacc ctcgatggca
1681gggtgaagct ctcggacttc ggattctgtg ctcagatcag caaagacgtc ccTAAGAGGA
1741AGTCCCTGGT GGGAACCCCC TACTGGATGG CTCCTGAAGT GATCTCCAGG TCTTTGTATG
1801CCACTGAGGT GGATATCTGG TCTCTGGGCA TCATGGTGAT TGAGATGGTA GATGGGGAGC
1861CACCGTACTT CAGTGACTCC CCAGTGCAAG CCATGAAGAG GCTCCGGGAC AGCCCCCCAC
1921CCAAGCTGAA AAACTCTCAC AAGGTCTCCC CAGTGCTGCG AGACTTCCTG GAGCGGATGC
1981TGGTGCGGGA CCCCCAAGAG AGAGCCACAG CCCAGGAGCT CCTAGACCAC CCCTTCCTGC
2041TGCAGACAGG GCTACCTGAG TGCCTGGTGC CCCTGATCCA GCTCTACCGA AAGCAGACCT
2101CCACCTGCTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
2161CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
2221TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAACTAGA CGAACTCCGT GGCATTGGAC
2281GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)