Construct: ORF TRCN0000480974
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007419.1_s317c1
- Derived from:
- ccsbBroadEn_05639
- DNA Barcode:
- TATACCGTTTCTAGTAAATTTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C1orf226 (400793)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480974
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 400793 | C1orf226 | chromosome 1 open reading f... | NM_001085375.2 | 100% | 100% | |
| 2 | human | 400793 | C1orf226 | chromosome 1 open reading f... | NM_001135240.2 | 86.3% | 86.3% | 1_129del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 885
- ORF length:
- 816
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtttgagaat ttgaacacag ccctcactcc aaagctccag gccagccgct 121 ccttccccca cttgtccaag cccgtggccc ccggctctgc ccctctgggc tctggtgagc 181 ctggggggcc aggactctgg gtgggcagca gccagcacct caagaacctg ggcaaagcca 241 tgggggccaa agtgaatgac ttcctgagga gaaaggagcc ctccagcctg ggcagtgtgg 301 gtgtgacaga gatcaacaag actgcaggag cacagctggc cagtgggact gacgcggctc 361 cagaggcttg gctagaggat gaaaggtcag tcctgcaaga aacatttcct cggctggatc 421 ctccacctcc cataaccaga aagcgaaccc ctcgggccct gaagaccacc caggacatgc 481 tgatttcatc acagcctgtc cTCAGCAGTC TGGAGTATGG GACAGAGCCA TCACCTGGGC 541 AGGCCCAGGA CTCCGCTCCC ACTGCCCAGC CTGACGTCCC AGCAGACGCT TCACAGCCAG 601 AGGCCACCAT GGAAAGAGAA GAGAGAGGCA AAGTTCTGCC CAATGGAGAG GTTTCCCTGT 661 CAGTACCTGA CCTAATCCAC AAGGATAGCC AGGACGAATC CAAGCTAAAG ATGACTGAGT 721 GCAGAAGGGC CTCCTCCCCC AGCCTTATCG AGAGGAATGG CTTCAAACTC AGCTTGAGCC 781 CCATCAGCCT GGCTGAGTCC TGGGAGGATG GCAGCCCCCC TCCTCAGGCA CGGACCTCCA 841 GCCTCGACAA TGAGGGCCCT CACCCAGACC TGCTGTCCTT TGAATTGCCA ACTTTCTTGT 901 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 961 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1021 GAAAGGACGA TATACCGTTT CTAGTAAATT TTTTACGCGT TAAGTCgaca atcaacctct 1081 ggattacaaa atttgtgaaa gatt