Construct: ORF TRCN0000480990
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000051.1_s317c1
- Derived from:
- ccsbBroadEn_13600
- DNA Barcode:
- GCCCTCGGATTGCCTATTCATACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NHSL2 (340527)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480990
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 340527 | NHSL2 | NHS like 2 | XM_017029477.1 | 82.4% | 82.5% | 2127_2577delinsT |
| 2 | human | 340527 | NHSL2 | NHS like 2 | XM_017029476.1 | 75.6% | 75.6% | 1_234del;2361_2811delinsT |
| 3 | human | 340527 | NHSL2 | NHS like 2 | XM_017029475.1 | 75.2% | 75.2% | 1_249del;2376_2826delinsT |
| 4 | human | 340527 | NHSL2 | NHS like 2 | XM_011530934.2 | 68.2% | 68.3% | 1_537del;2664_3114delinsT |
| 5 | human | 340527 | NHSL2 | NHS like 2 | XM_011530933.1 | 67.2% | 67.2% | 1_1035del |
| 6 | human | 340527 | NHSL2 | NHS like 2 | XM_011530932.1 | 64.5% | 64.5% | 1_1167del |
| 7 | human | 340527 | NHSL2 | NHS like 2 | XM_017029474.1 | 62.1% | 62.1% | 1_846del;2973_3423delinsT |
| 8 | human | 340527 | NHSL2 | NHS like 2 | XM_017029473.1 | 61.8% | 61.8% | 1_861del;2988_3438delinsT |
| 9 | human | 340527 | NHSL2 | NHS like 2 | NM_001013627.2 | 57.8% | 57.8% | 1_1098del;3225_3675delinsT |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2196
- ORF length:
- 2127
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagagcatg ggaatggtgt acagtgtccc cagttcttgc aatggaccta 121 cagaatcaac cttctccact tcctggaagg gagatgcttt tacctacatg actccaagtg 181 ccaccagcca gagcaatcaa gtcaatgaaa atgggaaaaa tccttcctgt gggaattctt 241 gggtctctct aaacaaagtc ccacctctgg ttcctaagga ggctgctacc ctccttgtcg 301 ctcgtgataa cccagcagga tgcagtgggt cagctggcta ccctgagcgc cttattcagc 361 aaaggcacat gcccgaaaga ccctccaaga ttggccttct gaccagtggg acctcgaggc 421 tggagacagg ccccggtggg gccagcagat tccgggagcg gtcactgtct gtgcccacag 481 actcaggcac cacagatgtg gactatgatg aggagcagaa ggccaatgag gcctgtgccc 541 tgccttttgc cagtacgagc tctgagggca gtaacagtgc tgacaacatt gcctccctta 601 gtgcccagca agaggcccag cacagaaggc agaggtccaa gagtatctca cttaggaagg 661 ccaaaaagaa gccttcccca cccacacgca gtgtctcact ggtcaaagat gagccaggcc 721 tcttgcctga aggtgggtca gcactaccca aggaccagag gcccaagagc ctttgcctct 781 ccttggaaca tcaaggacat cactcgtccc acccagatgc tcagggtcac ccagctattc 841 caaaccacaa agatccagaa agtacacaat tctcccacca ctggtatctt actgactgga 901 agtctggtga cacctaccaa tccctgtcca gctccagcac tgccactggc accacagtca 961 ttgagtgcac ccaagttcag ggcagctcag agtctcttgc ctcaccttcc acctccagag 1021 ccactacacc ttcccaactc tccattgaag tggaggccag ggagatatca tccccgggaa 1081 ggccccctgg actgatgtca ccctccagtg gctactccag ccagtcggaa acaccaacac 1141 ccactgtttc catgtccctg accctgggcc acttaccccc tccaagcagc agtgtccggg 1201 tacgtccagt ggtacctgag aggaagtcat cactaccccc gacgtcacca atggagaaat 1261 ttcccaagtc acggctatca tttgacctac cactgacctc ttcacccaac ctggatctgt 1321 ctgggatgag tatctccatc cgaagcaaaa ctaaggtgag tcggcaccac tcagagacaa 1381 attttggcgt caagctggcc cagaaaacta atcccaacca gccaatcatg cctatggtta 1441 ctcagtccga cctacgttct gttcgcctga ggtcggtcag caagtctgag ccggaagatg 1501 atattgagag ccctgagtat gccgaggaac ccagagcaga agaagtcttc accttgccag 1561 agagaaagac aaaacctccc gtagctgaga agcctccggt ggcccggagg cctccaagct 1621 tggtccacaa gccaccatct gttcctgagg agtatgcact aacttcacca accttggcta 1681 tgccccccag gagctcaatt caacatgcga gaccactccc tcaagacagc tacacggtag 1741 tgcggaaacc aaagccctcc agcttcccag atGGCAGAAG CCCAGGGGAG TCAACAGCAC 1801 CCTCATCTCT TGTTTTCACG CCTTTTGCCA GTTCCTCTGA TGCTTTCTTC TCAGGAACAC 1861 AGCAGCCTCC CCAGGGAAGT GTAGAGGACG AGGGCCCCAA GGTGAGGGTT CTGCCTGAAA 1921 GAATTAGCCT CCAGAGCCAG GAAGAAGCTG AGAAAAAGAA AGGCAAGATT CCACCTCCCG 1981 TACCAAAAAA ACCCAGCGTG CTGTACCTGC CTCTCACTTC TCCCACAGCT CAAATGGAGG 2041 CCTATGTGGC AGAACCAAGG CTGCCTCTCA GCCCCATCAT CACCCTGGAG GAAGACACCA 2101 AGTGTCCCGC CACCGGCGAT GACCTGCAAT CACTTGGTCA AAGGGTGACT TCAACTCCTC 2161 AGGCTGACAG TGAAAGGGAG GCAAGCCCTC TGGGTTTGCC AACTTTCTTG TACAAAGTGG 2221 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2281 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2341 AGCCCTCGGA TTGCCTATTC ATACGACGCG TTAAGTCgac aatcaacctc tggattacaa 2401 aatttgtgaa agatt