Construct: ORF TRCN0000481002
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011818.1_s317c1
- Derived from:
- ccsbBroadEn_04728
- DNA Barcode:
- GGCGATAGTGGCGGATACCTGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TWIST2 (117581)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481002
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 117581 | TWIST2 | twist family bHLH transcrip... | NM_001271893.4 | 100% | 100% | |
| 2 | human | 117581 | TWIST2 | twist family bHLH transcrip... | NM_057179.3 | 100% | 100% | |
| 3 | mouse | 13345 | Twist2 | twist basic helix-loop-heli... | NM_007855.3 | 96.6% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 546
- ORF length:
- 480
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggagggctcc agctcgcccg tgtcccccgt ggacagcctg ggcaccagcg 121 aggaggagct cgagaggcag cccaagcgct tcggccggaa gcggcgctac agcaagaagt 181 cgagcgaaga tggcagcccg accccgggca agcgcggCAA GAAGGGCAGC CCCAGCGCGC 241 AGTCCTTCGA GGAGCTGCAG AGCCAGCGCA TCCTGGCCAA CGTGCGCGAG CGCCAGCGCA 301 CCCAGTCGCT CAACGAGGCC TTCGCGGCGC TGCGCAAGAT CATCCCCACG CTGCCCTCTG 361 ACAAGCTGAG CAAGATCCAG ACGCTCAAGC TGGCCGCCAG GTACATAGAC TTCCTCTACC 421 AGGTCCTGCA GAGCGACGAG ATGGACAATA AGATGACCAG CTGCAGCTAC GTGGCCCACG 481 AGCGCCTCAG CTACGCCTTC TCCGTGTGGC GCATGGAGGG CGCGTGGTCC ATGTCCGCCT 541 CCCACTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 601 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 661 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGGCGATAGT GGCGGATACC TGCGCACGCG 721 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt