Construct: ORF TRCN0000481019
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004044.1_s317c1
- Derived from:
- ccsbBroadEn_06841
- DNA Barcode:
- CCGTGAGATAAGAAGATACACGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAP2B (5912)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481019
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5912 | RAP2B | RAP2B, member of RAS oncoge... | NM_002886.4 | 99.8% | 99.4% | 15A>T |
| 2 | human | 5911 | RAP2A | RAP2A, member of RAS oncoge... | NM_021033.7 | 83.5% | 90.1% | (many diffs) |
| 3 | mouse | 74012 | Rap2b | RAP2B, member of RAS oncoge... | NM_028712.4 | 93.2% | 99.4% | (many diffs) |
| 4 | mouse | 76108 | Rap2a | RAS related protein 2a | NM_029519.3 | 83% | 90.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 615
- ORF length:
- 549
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag agagtacaat gtggtggtgc tgggctcggg cggcgtgggc aagtccgcgc 121 tcaccgtgca gttcgtgacg ggctccTTCA TCGAGAAGTA CGACCCGACC ATCGAAGACT 181 TTTACCGCAA GGAGATTGAG GTGGACTCGT CGCCGTCGGT GCTGGAGATC CTGGATACGG 241 CGGGCACCGA GCAGTTCGCG TCCATGCGGG ACCTGTACAT CAAGAACGGC CAGGGCTTCA 301 TCCTGGTCTA CAGCCTCGTC AACCAGCAGA GCTTCCAGGA CATCAAGCCC ATGCGGGACC 361 AGATCATCCG CGTGAAGCGG TACGAGCGCG TGCCCATGAT CCTGGTGGGC AACAAGGTGG 421 ACCTGGAGGG TGAGCGCGAG GTCTCGTACG GGGAGGGCAA GGCCCTGGCT GAGGAGTGGA 481 GCTGCCCCTT CATGGAGACG TCGGCCAAAA ACAAAGCCTC GGTAGACGAG CTATTTGCCG 541 AGATCGTGCG GCAGATGAAC TACGCGGCGC AGCCCAACGG CGATGAGGGC TGCTGCTCGG 601 CCTGCGTGAT CCTCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 661 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 721 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CCGTGAGATA AGAAGATACA 781 CGAGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt