Construct: ORF TRCN0000481026
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018171.2_s317c1
- Derived from:
- ccsbBroadEn_00298
- DNA Barcode:
- TGCTATGCGCCGCAGGCACGAACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHAD (1101)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481026
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1101 | CHAD | chondroadherin | NM_001267.3 | 100% | 100% | |
| 2 | human | 1101 | CHAD | chondroadherin | XM_011524214.2 | 81.5% | 81.5% | 1_243del |
| 3 | mouse | 12643 | Chad | chondroadherin | NM_007689.4 | 86.3% | 93% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1143
- ORF length:
- 1077
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ccgcccaatg ctcttgctca gcctcggcct cctggctggt ctgctgccgg 121 cgctggccgc ctgcccccag aactgccact gccacagcga cctgcagcac gtcatctgcg 181 acaaggtggg gctgcagaag atccccaagg tgtcagagaa gaccaagctg ctcaacctac 241 agcgcaacaa cttcccggtg ctggctgcca attcgttccg ggccatgccg aacctcgtgt 301 cattgcacct gcagcactgc cagatccgcg aggtggccgc cggtgccttc cgcggcctca 361 agcaacttat ctacttgtac ctgtcccata acgacatccg cgtgctgcgc gcaggtgcct 421 tcgacgacct gaccgagctg acctacctct acctggacca caacaaggtc actgagctgc 481 cccgggggtt gctctccccg ctggtcaacc tcttcatctt gcagctcaac aacaacaaga 541 tccgtgagct gcgcgcaggc gccttccagg gagccaagga cctgcgctgg ctctacctgt 601 cggaaaacgc gttgagctcc ctgcagcccg gggccctgga cgacgtggag aacctcgcca 661 aattccacgt ggacaggaac cagctgtcca gctacccctc agctgcccTG AGCAAGCTAC 721 GGGTGGTGGA GGAGCTGAAG CTGTCCCACA ACCCCCTGAA AAGCATCCCG GACAATGCCT 781 TCCAGTCCTT TGGCAGATAC CTGGAGACCC TCTGGCTGGA CAACACCAAC CTGGAGAAGT 841 TCTCAGATGG TGCCTTCCTG GGTGTAACCA CGCTGAAACA CGTCCATTTG GAGAACAACC 901 GCTTGAACCA GCTACCCTCC AACTTCCCCT TCGACAGCCT GGAGACCCTC GCCCTTACCA 961 ATAACCCCTG GAAGTGTACC TGCCAGCTCC GGGGCCTTCG GCGGTGGCTG GAAGCCAAGG 1021 CCTCCCGCCC AGATGCCACC TGTGCCTCAC CTGCCAAGTT CAAGGGCCAG CACATCCGTG 1081 ACACGGACGC CTTCCGCAGC TGCAAGTTCC CCACCAAGAG GTCCAAGAAA GCTGGCCGCC 1141 ATTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1201 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1261 GGCTTTATAT ATCTTGTGGA AAGGACGATG CTATGCGCCG CAGGCACGAA CCACGCGTTA 1321 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt