Construct: ORF TRCN0000481057
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003356.1_s317c1
- Derived from:
- ccsbBroadEn_10828
- DNA Barcode:
- AACTGATTTAAATTTTCTAGAGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADAM2 (2515)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481057
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2515 | ADAM2 | ADAM metallopeptidase domain 2 | NM_001278113.2 | 100% | 100% | |
| 2 | human | 2515 | ADAM2 | ADAM metallopeptidase domain 2 | NM_001464.5 | 97.4% | 97.4% | 513_569del |
| 3 | human | 2515 | ADAM2 | ADAM metallopeptidase domain 2 | XM_005273468.1 | 93.3% | 93.3% | 513_569del;1613_1614ins90 |
| 4 | human | 2515 | ADAM2 | ADAM metallopeptidase domain 2 | NM_001278114.2 | 88.8% | 88.8% | 513_569del;1608_1609ins189 |
| 5 | human | 2515 | ADAM2 | ADAM metallopeptidase domain 2 | XM_011544479.1 | 85% | 85% | 512_513ins321 |
| 6 | human | 2515 | ADAM2 | ADAM metallopeptidase domain 2 | XM_011544480.1 | 80.8% | 80.8% | 512_513ins321;1235_1236ins90 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2214
- ORF length:
- 2148
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg gcgcgtcttg tttctgctca gcgggctcgg cgggctgcgg atggacagta 121 attttgatag tttacctgtg caaattacag ttccggagaa aatacggtca ataataaagg 181 aaggaattga atcgcaggca tcctacaaaa ttgtaattga agggaaacca tatactgtga 241 atttaatgca aaaaaacttt ttaccccata attttagagt ttacagttat agtggcacag 301 gaattatgaa accacttgac caagattttc agaatttctg ccactaccaa gggtatattg 361 aaggttatcc aaaatctgtg gtgatggtta gcacatgtac tggactcagg ggcgtactac 421 agtttgaaaa tgttagttat ggaatagaac ccctggagtc ttcagttggc tttgaacatg 481 taatttacca agtaaaacat aagaaagcag atgtttcctt atataatgag aaggatattg 541 aatcaagaga tctgtccttt aaattacaaa gcgtagagta taatcatatg gggtctgata 601 caactgttgt cgctcaaaaa gttttccagt tgattggatt gacgaatgct atttttgttt 661 catttaatat tacaattatt ctgtcttcat tggagctttg gatagatgaa aataaaattg 721 caaccactgg agaagctaat gagttattac acacattttt aagatggaaa acatcttatc 781 ttgttttacg tcctcatgat gtggcatttt tacttgttta cagagaaaag tcaaattatg 841 ttggtgcaac ctttcaaggg aagatgtgtg atgcaaacta tgcaggaggt gttgttctgc 901 accccagaac cataagtctg gaatcacttg cagttatttt agctcaatta ttgagcctta 961 gtatggggat cacttatgat gacattaaca aatgccagtg ctcaggagct gtctgcatta 1021 tgaatccaga agcaattcat ttcagtggtg tgaagatctt tagtaactgc agcttcgaag 1081 actttgcaca ttttatttca aagcagaagt cccagtgtct tcacaatcag cctcgcttag 1141 atcctttttt caaacagcaa gcagtgtgtg gtaatgcaaa gctggaagca ggagaggagt 1201 gtgactgtgg gactgaacag gattgtgccc ttattggaga aacatgctgt gatattgcca 1261 catgtagatt taaagccggt tcaaactgtg ctgaaggacc atgctgcgaa aactgtctat 1321 ttatgtcaaa agaaagaatg tgtaggcctt cctttgaaga atgcgacctc cctgaatatt 1381 gcaatggatc atctgcatca tgcccagaaa accactatgt tcagactggg catccgtgtg 1441 gactgaatca atggatctgt atagatggag tttgtatgag tggggataaa caatgtacag 1501 acacatttgg caaagaagta gagtttggcc cttcagaatg ttattctcac cttaattcaa 1561 agactgatgt atctggaaac tgtggtataa gtgattcagg atacacacag tgtgaagctg 1621 acaatctgca gtgcggaaaa ttaatatgta aatatgtagg taaattttta ttacaaattc 1681 caagagccac tattatttat gccaacataa gtggacatct ctgcattgct gtggaatttg 1741 ccagtgatca tgcagacagc caaaagatgt ggataaaaga tggaacttct tgtggttcaa 1801 ataaggttTG CAGGAATCAA AGATGTGTGA GTTCTTCATA CTTGGGTTAT GATTGTACTA 1861 CTGACAAATG CAATGATAGA GGTGTATGCA ATAACAAAAA GCACTGTCAC TGTAGTGCTT 1921 CATATTTACC TCCAGATTGC TCAGTTCAAT CAGATCTATG GCCTGGTGGG AGTATTGACA 1981 GTGGCAATTT TCCACCTGTA GCTATACCAG CCAGACTCCC TGAAAGGCGC TACATTGAGA 2041 ACATTTACCA TTCCAAACCA ATGAGATGGC CATTTTTCTT ATTCATTCCT TTCTTTATTA 2101 TTTTCTGTGT ACTGATTGCT ATAATGGTGA AAGTTAATTT CCAAAGGAAA AAATGGAGAA 2161 CTGAGGACTA TTCAAGCGAT GAGCAACCTG AAAGTGAGAG TGAACCTAAA GGGAACCCAA 2221 CCTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2281 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2341 TATCTTGTGG AAAGGACGAA ACTGATTTAA ATTTTCTAGA GTTACGCGTT AAGTCgacaa 2401 tcaacctctg gattacaaaa tttgtgaaag att