Construct: ORF TRCN0000481062
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000592.1_s317c1
- Derived from:
- ccsbBroadEn_00023
- DNA Barcode:
- TCCCGAGATTACATTTCAGACAAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ADA (100)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481062
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100 | ADA | adenosine deaminase | NM_000022.4 | 99.9% | 100% | 36G>A |
| 2 | human | 100 | ADA | adenosine deaminase | NM_001322051.1 | 93.3% | 93.3% | 604_605ins72 |
| 3 | human | 100 | ADA | adenosine deaminase | NR_136160.1 | 64.9% | (many diffs) | |
| 4 | human | 100 | ADA | adenosine deaminase | NM_001322050.1 | 62.8% | 55.3% | 0_1ins289;72_73ins116 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1155
- ORF length:
- 1089
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccagacgccc gccttcgaca agcccaaagt agaactgcat gtccacctag 121 acggatccat caagcctgaa accatcttat actatggcag gaggagaggg atcgccctcc 181 cagctaacac agcagagggg ctgctgaacg tcattggcat ggacaagccg ctcacccttc 241 cagacttcct ggccaagttt gactactaca tgcctgctat cgcgggctgc cgggaggcta 301 tcaaaaggat cgcctatgag tttgtagaga tgaaggccaa agagggcgtg gtgtatgtgg 361 aggtgcggta cagtccgcac ctgctggcca actccaaagt ggagccaatc ccctggaacc 421 aggctgaagg ggacctcacc ccagacgagg tggtggccct agtgggccag ggcctgcagg 481 agggggagcg agacttcggg gtcaaggccc ggtccatcct gtgctgcatg cgccaccagc 541 ccaactggtc ccccaaggtg gtggagctgt gtaagaagta ccagcagcag accgtggtag 601 ccattgacct ggctggagat gagaccatcc caggaagcag cctcttgcct ggacatgtcc 661 aggccTACCA GGAGGCTGTG AAGAGCGGCA TTCACCGTAC TGTCCACGCC GGGGAGGTGG 721 GCTCGGCCGA AGTAGTAAAA GAGGCTGTGG ACATACTCAA GACAGAGCGG CTGGGACACG 781 GCTACCACAC CCTGGAAGAC CAGGCCCTTT ATAACAGGCT GCGGCAGGAA AACATGCACT 841 TCGAGATCTG CCCCTGGTCC AGCTACCTCA CTGGTGCCTG GAAGCCGGAC ACGGAGCATG 901 CAGTCATTCG GCTCAAAAAT GACCAGGCTA ACTACTCGCT CAACACAGAT GACCCGCTCA 961 TCTTCAAGTC CACCCTGGAC ACTGATTACC AGATGACCAA ACGGGACATG GGCTTTACTG 1021 AAGAGGAGTT TAAAAGGCTG AACATCAATG CGGCCAAATC TAGTTTCCTC CCAGAAGATG 1081 AAAAGAGGGA GCTTCTCGAC CTGCTCTATA AAGCCTATGG GATGCCACCT TCAGCCTCTG 1141 CAGGGCAGAA CCTCTGACCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1201 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1261 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TCCCGAGATT ACATTTCAGA 1321 CAATACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt