Construct: ORF TRCN0000481082
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010404.2_s317c1
- Derived from:
- ccsbBroadEn_04506
- DNA Barcode:
- ATTTTGCGGGACTGGGGTACCCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EFCAB11 (90141)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481082
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 90141 | EFCAB11 | EF-hand calcium binding dom... | NM_145231.4 | 100% | 100% | |
| 2 | human | 90141 | EFCAB11 | EF-hand calcium binding dom... | NM_001284266.2 | 86.3% | 84% | (many diffs) |
| 3 | human | 90141 | EFCAB11 | EF-hand calcium binding dom... | NM_001284269.2 | 85% | 84.6% | 1_1delAins70;3_4insGAA |
| 4 | human | 90141 | EFCAB11 | EF-hand calcium binding dom... | NM_001284268.2 | 71.4% | 68.7% | (many diffs) |
| 5 | human | 90141 | EFCAB11 | EF-hand calcium binding dom... | NM_001284267.1 | 70.5% | 70.5% | 0_1ins144 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 555
- ORF length:
- 489
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt cttctccgag gccagagcca ggTCGCGGAC GTGGGAAGCC AGTCCCTCGG 121 AACACAGGAA GTGGGTGGAA GTATTTAAAG CATGTGATGA AGATCACAAA GGATATCTCA 181 GCAGAGAGGA CTTTAAAACT GCTGTTGTAA TGCTGTTTGG GTACAAGCCC TCCAAGATAG 241 AAGTGGATTC TGTGATGTCT TCAATAAATC CAAATACTTC TGGTATATTA CTCGAGGGGT 301 TTTTAAATAT TGTCAGGAAA AAGAAGGAAG CTCAACGATA TCGGAACGAA GTAAGACACA 361 TCTTCACAGC CTTTGACACC TACTATCGTG GATTTTTAAC TTTGGAAGAT TTCAAAAAAG 421 CATTTAGGCA GGTGGCTCCC AAATTACCGG AAAGGACTGT TCTTGAGGTA TTCAGGGAAG 481 TAGATCGAGA TTCAGATGGT CACGTCAGCT TTAGAGACTT TGAATATGCC CTGAACTATG 541 GACAGAAGGA AGCCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 601 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 661 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ATTTTGCGGG ACTGGGGTAC 721 CCGTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt