Construct: ORF TRCN0000481090
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004492.1_s317c1
- Derived from:
- ccsbBroadEn_15654
- DNA Barcode:
- GACTTAATAATGTGACAGCCACTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNAJA3 (9093)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481090
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9093 | DNAJA3 | DnaJ heat shock protein fam... | NM_001286516.1 | 80.2% | 80.3% | 1_177del;624T>A |
| 2 | human | 9093 | DNAJA3 | DnaJ heat shock protein fam... | NM_001135110.3 | 53.1% | 53.2% | 1_636del;1083T>A |
| 3 | human | 9093 | DNAJA3 | DnaJ heat shock protein fam... | NM_005147.6 | 49.6% | 49.3% | (many diffs) |
| 4 | mouse | 83945 | Dnaja3 | DnaJ heat shock protein fam... | NM_001135112.1 | 46.7% | 49.8% | (many diffs) |
| 5 | mouse | 83945 | Dnaja3 | DnaJ heat shock protein fam... | NM_023646.4 | 44% | 46% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 789
- ORF length:
- 723
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gttgacattc aatcaagctg caaagggggt caacaaggag ttcaccgtga 121 acatcatgga cacgtgtgag cgctgcaacg gcaaggggaa cgagcccggc accaaggtgc 181 agcattgcca ctactgtggc ggctccggca tggaaaccat caacacaggc ccttttgtga 241 tgcgttccac gtgtaggaga tgtggtggcc gcggctccat catcatatcg ccctgtgtgg 301 tctgcagggg agcaggacaa gccaagcaga aaaagcgagt gatgatCCCT GTGCCTGCAG 361 GAGTCGAGGA TGGCCAGACC GTGAGGATGC CTGTGGGAAA AAGGGAAATT TTCATTACGT 421 TCAGGGTGCA GAAAAGCCCT GTGTTCCGGA GGGACGGCGC AGACATCCAC TCCGACCTCT 481 TTATTTCTAT AGCTCAGGCT CTTCTTGGGG GAACAGCCAG AGCCCAGGGC CTGTACGAGA 541 CGATCAACGT GACGATCCCC CCTGGGACTC AGACAGACCA GAAGATTCGG ATGGGTGGGA 601 AAGGCATCCC CCGGATTAAC AGCTACGGCT ACGGAGACCA CTACATCCAC ATCAAGATAC 661 GAGTTCCAAA GAGGCTAACG AGCCGGCAGC AGAGCCTGAT CCTGAGCTAC GCCGAGGACG 721 AGACAGATGT GGAGGGGACG GTGAACGGCG TCACCCTCAC CAGCTCTGGA AAAAGATCCA 781 CTGGAAACTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 841 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 901 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGACTTA ATAATGTGAC AGCCACTAAC 961 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt