Construct: ORF TRCN0000481099
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018411.1_s317c1
- Derived from:
- ccsbBroadEn_13555
- DNA Barcode:
- TTGTAGGTATAGCCTCCTCCCGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C5orf64 (285668)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481099
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285668 | C5orf64 | chromosome 5 open reading f... | NR_161251.1 | 12.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 459
- ORF length:
- 390
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg taCAAAAAAG 61 TTGGCACCAT GCTGGCACCA CTCTTCTTAT GCTGTCTGCG TAACTTGTTC AGAAAACTCA 121 TTAGCTTCCA ACCTCCTCAA TTAGGCAGAA CAAACATGCA TTACTCCAAA TTGCCCCGCA 181 CTGCGATAGA GACAGAGTTC AAGCAGAATG TTGGCCCACC ACCCAAGGAT CTGACCACTG 241 AAGTTTACTT CCCTTCAATC AAATCGAGAT CACATCTTCC TGCAGTTTTC TATAATCAGT 301 ATTTCAAGCA TCCAAAATGT GTAGGAGAAT ATGGACCCAA GAACGGAGCA GAGAGACAAA 361 TAGAGGAATG GAAAGTTTTG CCTACAACCA TGATGTTCTC AATGTTAGCT GACTGTGTTC 421 TAAAATCCAC TCCAATTCCC ATTTTGGGAG TTGCTATGTT GCCAACTTTC TTGTACAAAG 481 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 541 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 601 ACGATTGTAG GTATAGCCTC CTCCCGCGAC GCGTTAAGTC gacaatcaac ctctggatta 661 caaaatttgt gaaagatt