Construct: ORF TRCN0000481127
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018992.4_s317c1
- Derived from:
- ccsbBroadEn_14647
- DNA Barcode:
- AATGGACTAATAGTCCTTCCAAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FRK (2444)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481127
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2444 | FRK | fyn related Src family tyro... | NM_002031.3 | 100% | 100% | |
| 2 | human | 2444 | FRK | fyn related Src family tyro... | XM_005266880.4 | 100% | 100% | |
| 3 | human | 2444 | FRK | fyn related Src family tyro... | XM_005266881.2 | 100% | 100% | |
| 4 | human | 2444 | FRK | fyn related Src family tyro... | XM_005266882.4 | 100% | 100% | |
| 5 | human | 2444 | FRK | fyn related Src family tyro... | XM_011535653.2 | 100% | 100% | |
| 6 | human | 2444 | FRK | fyn related Src family tyro... | XM_011535654.2 | 100% | 100% | |
| 7 | human | 2444 | FRK | fyn related Src family tyro... | XM_011535655.2 | 100% | 100% | |
| 8 | human | 2444 | FRK | fyn related Src family tyro... | XM_017010645.1 | 100% | 100% | |
| 9 | human | 2444 | FRK | fyn related Src family tyro... | XM_011535656.2 | 77.5% | 77.2% | 0_1ins338;2delT;5_6insCC |
| 10 | mouse | 14302 | Frk | fyn-related kinase | NM_001159544.1 | 86% | 88.2% | (many diffs) |
| 11 | mouse | 14302 | Frk | fyn-related kinase | NM_010237.3 | 86% | 88.2% | (many diffs) |
| 12 | mouse | 14302 | Frk | fyn-related kinase | XM_006512534.3 | 86% | 88.2% | (many diffs) |
| 13 | mouse | 14302 | Frk | fyn-related kinase | XM_006512535.3 | 86% | 88.2% | (many diffs) |
| 14 | mouse | 14302 | Frk | fyn-related kinase | XM_006512536.3 | 86% | 88.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1584
- ORF length:
- 1515
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagcaacatc tgtcagaggc tctgggagta cctagaaccc tatctcccct 121 gtttgtccac ggaggcagac aagtcaaccg tgattgaaaa tccaggggcc ctttgctctc 181 cccagtcaca gaggcatggc cactactttg tggctttgtt tgattaccag gctcggactg 241 ctgaggactt gagcttccga gcaggtgaca aacttcaagt tctggacact ttgcatgagg 301 gctggtggtt tgccagacac ttggagaaaa gacgagatgg ctccagtcag caactacaag 361 gctatattcc ttctaactac gtggctgagg acagaagcct acaggcagag ccgtggttct 421 ttggagcaat cggaagatca gatgcagaga aacaactatt atattcagaa aacaagaccg 481 gttcctttct aatcagagaa agtgaaagcc aaaaaggaga attctctctt tcagttttag 541 atggagcagt tgtaaaacac tacagaatta aaagactgga tgaaggggga ttttttctca 601 cgcgaagaag aatcttttca acactgaacg aatttgtgag ccactacacc aagacaagtg 661 acggcctgtg tgtcaagctg gggaaaccat gcttaaagat ccaggtccca gctccatttg 721 atttgtcgta taaaaccgtg gaccaatggg agatagaccg caactccata cagcttctga 781 agcgattggg atctggtcag tttggcgaag tatgggaagg tctgtggaac aataccactc 841 cagtagcagt gaaaacatta aaaccaggtt caatggatcc aaatgacttc ctgagggagg 901 cacagataat gaagaaccta agacatccaa agcttatcca gctttatgct gtttgcactt 961 tagaagatcc aatttatatt attacagagt tgatgagaca tggaagtctg caagaatatc 1021 tccaaaatga cactggatca aaaatccatc tgactcaaca ggtagacatg gcggcacagg 1081 ttgcctctgg aatggcctat ctggagtctc ggaactaCAT TCACAGAGAT CTGGCTGCCA 1141 GAAATGTCCT CGTTGGTGAA CATAATATCT ACAAAGTAGC AGATTTTGGA CTTGCCAGAG 1201 TTTTTAAGGT AGATAATGAA GACATCTATG AATCTAGACA CGAAATAAAG CTGCCGGTGA 1261 AGTGGACTGC GCCCGAAGCC ATTCGTAGTA ATAAATTCAG CATTAAGTCC GATGTATGGT 1321 CATTTGGAAT CCTTCTTTAT GAAATCATTA CTTATGGCAA AATGCCTTAC AGTGGTATGA 1381 CAGGTGCCCA GGTAATCCAG ATGTTGGCTC AAAACTATAG ACTTCCGCAA CCATCCAACT 1441 GTCCACAGCA ATTTTACAAC ATCATGTTGG AGTGCTGGAA TGCAGAGCCT AAGGAACGAC 1501 CTACATTTGA GACACTGCGT TGGAAACTTG AAGACTATTT TGAAACAGAC TCTTCATATT 1561 CAGATGCAAA TAACTTCATA AGACCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1621 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1681 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAATG GACTAATAGT 1741 CCTTCCAAAG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt