Construct: ORF TRCN0000481133
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016075.1_s317c1
- Derived from:
- ccsbBroadEn_06069
- DNA Barcode:
- AGAACTATATCTCGGACACGCACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYB561 (1534)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481133
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1534 | CYB561 | cytochrome b561 | NM_001017916.1 | 99.8% | 99.6% | 16G>T |
| 2 | human | 1534 | CYB561 | cytochrome b561 | NM_001017917.2 | 99.8% | 99.6% | 16G>T |
| 3 | human | 1534 | CYB561 | cytochrome b561 | NM_001915.4 | 99.8% | 99.6% | 16G>T |
| 4 | human | 1534 | CYB561 | cytochrome b561 | NM_001330421.2 | 97.1% | 96.8% | 1_21del;37G>T |
| 5 | mouse | 13056 | Cyb561 | cytochrome b-561 | NM_007805.4 | 84.8% | 88.4% | (many diffs) |
| 6 | mouse | 13056 | Cyb561 | cytochrome b-561 | XM_006532130.3 | 84.8% | 88.4% | (many diffs) |
| 7 | mouse | 13056 | Cyb561 | cytochrome b-561 | XM_006532131.2 | 84.8% | 88.4% | (many diffs) |
| 8 | mouse | 13056 | Cyb561 | cytochrome b-561 | XM_006532132.3 | 84.8% | 88.4% | (many diffs) |
| 9 | mouse | 13056 | Cyb561 | cytochrome b-561 | XM_006532134.3 | 57.2% | 51.7% | (many diffs) |
| 10 | mouse | 13056 | Cyb561 | cytochrome b-561 | XM_006532133.3 | 55.1% | 49.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 819
- ORF length:
- 753
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gggcggggcc tcggcagcca cccccacagc actgccttac tacgtggcct 121 tctcccagct gctgggcctg accttggtgg ccatgaccgg cgcgtggctc gggctgtacc 181 gaggcggcat tgcctgggag agcgacctgc agttcaacgc gcaccccctc tgcatggtca 241 taggcctgat cttcctgcag ggaaatgccc tgctggttta ccgtgtcttc aggaacgaag 301 ctaaacgcac caccaaggtc ctgcacgggc tgctgcacaT CTTTGCGCTC GTCATCGCCC 361 TGGTTGGCTT GGTGGCGGTG TTCGACTACC ACAGGAAGAA GGGCTACGCT GACCTGTACA 421 GCCTACACAG CTGGTGCGGG ATCCTTGTCT TTGTCCTGTA CTTTGTGCAG TGGCTGGTGG 481 GCTTCAGCTT CTTCCTGTTC CCCGGAGCTT CATTCTCCCT GCGGAGCCGC TACCGCCCAC 541 AGCACATCTT CTTTGGTGCT ACCATCTTCC TCCTTTCCGT GGGCACCGCC CTGCTGGGCC 601 TGAAGGAGGC ACTGCTGTTC AACCTCGGGG GCAAGTATAG CGCATTTGAG CCCGAGGGTG 661 TCCTGGCCAA CGTGCTGGGC CTGCTGCTGG CCTGCTTCGG TGGGGCGGTG CTCTACATCT 721 TGACCCGGGC CGACTGGAAG CGGCCTTCCC AGGCGGAAGA GCAGGCCCTC TCCATGGACT 781 TCAAGACGCT GACGGAGGGA GATAGCCCCG GCTCCCAGTG CCCAACTTTC TTGTACAAAG 841 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 901 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 961 ACGAAGAACT ATATCTCGGA CACGCACCAC GCGTTAAGTC gacaatcaac ctctggatta 1021 caaaatttgt gaaagatt