Construct: ORF TRCN0000481138
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014989.1_s317c1
- Derived from:
- ccsbBroadEn_14410
- DNA Barcode:
- ATGCGATGACCGCCACCACATATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NSUN5P1 (155400)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481138
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_001168348.2 | 28.4% | 27.6% | (many diffs) |
| 2 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_018044.4 | 25.9% | 25.1% | (many diffs) |
| 3 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_148956.4 | 23.8% | 23.1% | (many diffs) |
| 4 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_001168347.3 | 23.6% | 22.9% | (many diffs) |
| 5 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | XR_242255.5 | 20.5% | (many diffs) | |
| 6 | human | 260294 | NSUN5P2 | NSUN5 pseudogene 2 | NR_033323.3 | 19.5% | 1_1112del;1136G>T;1463_1784delinsG | |
| 7 | human | 155400 | NSUN5P1 | NSUN5 pseudogene 1 | NR_033322.3 | 19.4% | 1_1119del;1295C>A;1469_1786delinsCG | |
| 8 | human | 155400 | NSUN5P1 | NSUN5 pseudogene 1 | NR_104013.1 | 18.4% | (many diffs) | |
| 9 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | XM_005277604.5 | 15.9% | 6.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 420
- ORF length:
- 351
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccgagcaga cagctggagg atcccggggc agggacacct agcccggtgc 121 gtctgcatgc CCTGGCAGGG TTCCAGCAGC GAGCCCTGTG CCACGCGCTC ACTTTCCCTT 181 CCCTGCAGCG GCTCGTCTAC TCCATGTGCT CCCTCTGCCA GGAGGAGAAT GAAGACATGG 241 TACAAGATGC GCTGCAGCAG AACCCGGGCG CCTTCAGGCT AGCTCCCGCC CTGCCTGCCC 301 GGCCCCACCG AGGCCTGAGC ACGTTCCCGG GTGCCGAGCA CTGCCTCCGG GCTTCCCCCA 361 AGACCACGCT TAGCGGTGGC TTCTTCGTTG CTGTAATTGA ACGGGTCGAG ATGCCGACGT 421 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 481 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 541 TTTATATATC TTGTGGAAAG GACGAATGCG ATGACCGCCA CCACATATCA CGCGTTAAGT 601 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt