Construct: ORF TRCN0000481153
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000640.1_s317c1
- Derived from:
- ccsbBroadEn_07741
- DNA Barcode:
- CTTAATTACAAGATTCTCTGTCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAPGEF4 (11069)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481153
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11069 | RAPGEF4 | Rap guanine nucleotide exch... | NM_001100397.1 | 99.8% | 99.5% | (many diffs) |
| 2 | human | 11069 | RAPGEF4 | Rap guanine nucleotide exch... | NM_001282899.1 | 98.7% | 98.5% | (many diffs) |
| 3 | human | 11069 | RAPGEF4 | Rap guanine nucleotide exch... | XM_017003197.1 | 97.7% | 97.4% | (many diffs) |
| 4 | human | 11069 | RAPGEF4 | Rap guanine nucleotide exch... | NM_001282900.1 | 96.6% | 96.4% | (many diffs) |
| 5 | human | 11069 | RAPGEF4 | Rap guanine nucleotide exch... | XM_006712205.3 | 96.6% | 96.4% | (many diffs) |
| 6 | human | 11069 | RAPGEF4 | Rap guanine nucleotide exch... | XM_011510505.3 | 96.5% | 91.6% | (many diffs) |
| 7 | human | 11069 | RAPGEF4 | Rap guanine nucleotide exch... | NM_001282901.1 | 91% | 90.7% | (many diffs) |
| 8 | human | 11069 | RAPGEF4 | Rap guanine nucleotide exch... | NM_007023.4 | 85.4% | 85% | (many diffs) |
| 9 | human | 11069 | RAPGEF4 | Rap guanine nucleotide exch... | XM_005246246.3 | 83.6% | 83.2% | (many diffs) |
| 10 | human | 11069 | RAPGEF4 | Rap guanine nucleotide exch... | XM_017003196.2 | 80% | 79.2% | (many diffs) |
| 11 | mouse | 56508 | Rapgef4 | Rap guanine nucleotide exch... | NM_001204166.1 | 91.5% | 96.7% | (many diffs) |
| 12 | mouse | 56508 | Rapgef4 | Rap guanine nucleotide exch... | XM_017319138.1 | 89.5% | 94.8% | (many diffs) |
| 13 | mouse | 56508 | Rapgef4 | Rap guanine nucleotide exch... | XM_017319139.1 | 88.6% | 93.8% | (many diffs) |
| 14 | mouse | 56508 | Rapgef4 | Rap guanine nucleotide exch... | XM_011239711.2 | 88.2% | 92.7% | (many diffs) |
| 15 | mouse | 56508 | Rapgef4 | Rap guanine nucleotide exch... | XM_006499938.3 | 83.2% | 88.3% | (many diffs) |
| 16 | mouse | 56508 | Rapgef4 | Rap guanine nucleotide exch... | NM_001204165.1 | 78.4% | 82.7% | (many diffs) |
| 17 | mouse | 56508 | Rapgef4 | Rap guanine nucleotide exch... | NM_019688.2 | 76.7% | 81.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2667
- ORF length:
- 2601
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct ctacaagaaa tatcgacagt atatggcagg acttctggct cctccttatg 121 gtgttatgga aacgggctct aacaatgaca ggattcctga caaggagaac acacctctca 181 ttgaacctca cgttcctctt cgtcctgcta acaccattac caaggtccct tcagagaaga 241 tcctcagagc tggaaaaatt ttacgaaatg ccattctctc tcgagcacct cacatgataa 301 gagatagaaa ataccaccta aagacataca gacaatgctg tgtgggaact gaactggtgg 361 actggatgat gcagcagaca ccatgtgttc actcccggac tcaagctgtt ggcatgtggc 421 aagtcctgtt agaagatggt gttctcaacc acgtggacca ggagcaccat ttccaagaca 481 aatatttatt ctatcgattt ctggatgatg agcacgagga tgcccctttg cctactgagg 541 aggagaagaa ggagtgtgat gaggagctcc aggacaccat gctgctgctg tcacagatgg 601 gccgcgacgc ccacatgagg atgatccttc gcaaaccacc tggccagagg actgtggatg 661 acctagagat tatctatgag gagcttcttc atattaaagc cttatcccat ctttctacca 721 cagtgaaacg agagttagca ggtgttctca tttttgagtc tcacgccaaa ggagggactg 781 tgttgtttaa ccagggggaa gaaggtacct cctggtacat tattctaaaa ggatcagtga 841 atgtagtcat ttacggcaag ggtgtggtct gcaccctgca tgaaggagat gacttcggca 901 agttagcact agtgaatgat gccccacgag ctgcctctat cgtcttacga gaagataact 961 gccatttctt aagagtagac aaggaggatt tcaaccggat cctaagggac gtggaggcga 1021 atacagtcag acttaaagaa catgaccaag atgtcttggt gctggagaag gtcccagcag 1081 ggaacagagc ttctaatcaa ggaaactcac agcctcagca aaagtatact gtgatgtcag 1141 gaacacctga aaaaatttta gagcgttttc tagaaacaat acgccttgag gcaactttaa 1201 atgaagcaac agattctgtt ttaaatgact ttattatgat gcactgtgtt tttatgccaa 1261 atacccagct ttgcccggca ctggtggccc actaccacgc acagccttca caaggtacag 1321 aacaggagaa aatggattat gccctcaaca ataagaggcg agtcatccgc ctggttctac 1381 agtgggctgc catgtatgga gacctcctgc aagaggatga cgtgtctatg gccttcctgg 1441 aggagtttta tgtatctgta tcagatgatg cccggatgat tgctgccctc aaggagcaac 1501 tgccagagtt ggagaagatt gtcaagcaaa tctcagaaga tgcaaaggca ccacaaaaga 1561 agcacaaggt tattttgcaa cagttcaata cgggcgatga gagagcccag aagcgccagc 1621 ctatccgcgg ctctgatgaa gttctgttta aggtctattg catggaccac acctacacaa 1681 ccattcgggt gccagtggcc acttcggtga aggaagtcat cagtgcagtt gccgacaagc 1741 tgggctccgg ggagggcctg atcatagtca agatgagttc cggaggagaa aaggtggtgc 1801 tcaaacctaa tgatgtttca gtatttacga cgctcaccat taatggacgc ctgtttgctt 1861 gcccgcgaga gcaattcgat tcactgactc cattaccaga acaggaaggc ccaactgttg 1921 gaacagtggg aacttttgaa ctgatgagct ccaaagattt agcataccag atgacaattt 1981 atgattggga actcttcaac tgcgtgcatg agctggagct aatctatcac acatttggaa 2041 ggcataattt taaaaagacc acagcaaact tggatttgtt cctgaggaga tttaatgaaa 2101 ttcagttttg ggtcgtcact gagatctgcc tttgttctca gctcagcaag cgtgttcagc 2161 tattaaaaaa atttattaag atagcagccc actgtaagga gtataaaaaT CTGAATTCCT 2221 TTTTTGCCAT CGTCATGGGA CTAAGTAACG TTGCTGTGAG CCGCTTGGCA CTAACGTGGG 2281 AGAAACTGCC AAGCAAGTTC AAGAAGTTCT ATGCGGAGTT TGAAAGTTTA ATGGACACTT 2341 CAAGGAACCA CAGGGCCTAC AGGCTGACAG TAGCTAAGCT GGAACCTCCT CTCATCCCCT 2401 TCATGCCTTT GCTCATTAAA GATATGACAT TTACTCATGA GGGGAACAAG ACGTTCATTG 2461 ACAATCTAGT AAACTTTGAA AAAATGCGCA TGATTGCAAA TACGGCCAGA ACAGTGAGAT 2521 ACTACAGGAG CCAACCCTTC AATCCTGATG CAGCTCAAGC TAATAAGAAC CATCAGGATG 2581 TCCGGAGTTA TGTACGGCAA TTAAATGTGA TTGACAACCA GAGAACTTTA TCACAGATGT 2641 CACACAGATT AGAGCCTCGT CGACCATACC CAACTTTCTT GTACAAAGTG GTTGATATCG 2701 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2761 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACTTAATTA 2821 CAAGATTCTC TGTCCTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2881 aagatt