Construct: ORF TRCN0000481164
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012162.1_s317c1
- Derived from:
- ccsbBroadEn_09171
- DNA Barcode:
- ATTACCTTTGTGAACAGCTCCAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COQ5 (84274)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481164
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84274 | COQ5 | coenzyme Q5, methyltransferase | NM_032314.4 | 99.8% | 100% | 24T>C |
| 2 | human | 84274 | COQ5 | coenzyme Q5, methyltransferase | XM_006719639.2 | 75.2% | 75.2% | 0_1ins243 |
| 3 | human | 84274 | COQ5 | coenzyme Q5, methyltransferase | XM_024449224.1 | 75.2% | 75.2% | 0_1ins243 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1050
- ORF length:
- 981
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggccccc gggagctgtg ccctatggag ctattgcggc cgtgggtggt 121 cgcgggcgat gcggggctgc cagctcctcg ggcttcgtag ctcttggccc ggggacctac 181 taagtgctcg gctcttgtcc caagagaagc gggcagcgga aacgcacttt gggtttgaga 241 ctgtgtcgga agaggagaag gggggcaaag tctatcaggt gtttgaaagt gtggctaaga 301 agtatgatgt gatgaatgat atgatgagtc ttggtatcca tcgtgtttgg aaggatttgc 361 tgctctggaa gatgcacccg cttcctggga cccagctgct tgatgttgct ggaggcacag 421 gtgacattgc attccggttc cttaattatg ttcagtccca gcatcagaga aaacagaaga 481 ggcagttaag ggcccaacaa aatttatcct gggaagaaat tgccaaagag taccagaatg 541 aagaagattc cttgggcggg tctcgtgtcg tggtgtgtga catcaacaag gagatgctaa 601 aggttggaaa gcagaaagCC TTGGCTCAAG GATACAGAGC TGGACTTGCA TGGGTATTAG 661 GAGATGCTGA AGAACTGCCC TTTGATGATG ACAAGTTTGA TATTTACACC ATTGCCTTTG 721 GGATCCGGAA TGTCACACAC ATTGATCAGG CACTCCAGGA AGCTCATCGG GTGCTGAAAC 781 CAGGAGGACG GTTTCTCTGT CTGGAATTTA GCCAAGTGAA CAATCCCCTC ATATCCAGGC 841 TTTATGATCT ATATAGCTTC CAGGTCATCC CTGTCCTGGG AGAGGTCATC GCTGGAGACT 901 GGAAGTCCTA TCAGTACCTT GTAGAGAGTA TCCGAAGGTT TCCGTCTCAG GAAGAGTTCA 961 AGGACATGAT AGAAGATGCA GGCTTTCACA AGGTGACTTA CGAAAGTCTA ACATCAGGCA 1021 TTGTGGCCAT TCATTCTGGC TTCAAACTTT TGCCAACTTT CTTGTACAAA GTGGTTGATA 1081 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1141 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAATTAC 1201 CTTTGTGAAC AGCTCCAACA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1261 tgaaagatt