Construct: ORF TRCN0000481169
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008871.1_s317c1
- Derived from:
- ccsbBroadEn_08149
- DNA Barcode:
- GCCCCATGGAAAATAACTTCGGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POMT2 (29954)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481169
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29954 | POMT2 | protein O-mannosyltransfera... | NM_013382.5 | 99.9% | 99.8% | 162G>T;172T>N |
| 2 | human | 29954 | POMT2 | protein O-mannosyltransfera... | XM_011536675.2 | 92% | 92.1% | (many diffs) |
| 3 | human | 29954 | POMT2 | protein O-mannosyltransfera... | XM_011536676.2 | 78.5% | 78.5% | (many diffs) |
| 4 | human | 29954 | POMT2 | protein O-mannosyltransfera... | XM_011536677.3 | 73.2% | 73.3% | (many diffs) |
| 5 | human | 29954 | POMT2 | protein O-mannosyltransfera... | XR_943416.3 | 45.6% | (many diffs) | |
| 6 | human | 29954 | POMT2 | protein O-mannosyltransfera... | XR_001750279.1 | 45.2% | (many diffs) | |
| 7 | human | 29954 | POMT2 | protein O-mannosyltransfera... | XR_001750282.1 | 40% | (many diffs) | |
| 8 | mouse | 217734 | Pomt2 | protein-O-mannosyltransfera... | NM_153415.4 | 81.1% | 82.9% | (many diffs) |
| 9 | mouse | 217734 | Pomt2 | protein-O-mannosyltransfera... | XM_011244088.2 | 79.4% | 81.2% | (many diffs) |
| 10 | mouse | 217734 | Pomt2 | protein-O-mannosyltransfera... | XM_011244089.2 | 55.1% | 55.1% | (many diffs) |
| 11 | mouse | 217734 | Pomt2 | protein-O-mannosyltransfera... | XM_011244090.2 | 48.4% | 48.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2316
- ORF length:
- 2250
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gccggccacg ggcggaggcc tggcagagtc cgagctgcgt ccccggaggg 121 gccgctgtgg cccccaggct gctagggccg caggccggga cgtggccgct gaggctgtgg 181 cgcgaagccc caaacggcct gcttggggct cacggcgctt cgaggctgtc ggctggnggg 241 ccctgctggc cttggtgacg ctgctgtcct tcgccacccg cttccaccgc ttggacgagc 301 cgccgcacat ctgttgggat gagactcact ttggaaaaat gggaagttac tatatcaacc 361 gtacattttt ctttgatgtg cacccgcccc tgggaaagat gctgataggt cttgctggct 421 acctgagtgg atatgatggt acctttttgt tccagaagcc tggggataaa tatgagcatc 481 acagctacat gggaatgaga ggattctgtg cattccttgg ctcctggctg gtcccctttg 541 cctacctcac tgtactggat ctgtccaagt ccctctcggc agcactgctc acagctgccc 601 tcctcacctt tgacacggga tgcctcactc tgtcccagta catcctcctt gaccccatcc 661 tgatgttctt catcatggct gccatgctga gcatggtcaa gtacaactct tgcgccgaca 721 ggcccttctc tgccccctgg tggttctggc tcagcctgac tggcgttagt cttgctggtg 781 ctttaggggt caagtttgtt ggcctcttta tcatccttca agtggggctg aacaccattg 841 cagacctttg gtacctgttc ggagacctca gtctttcatt ggtgactgtg ggaaaacacc 901 tgactgctcg tgtcctgtgc ctcatagtgc tgcccctggc tctctataca gccacctttg 961 ctgttcactt catggtgctg agtaaaagtg gccctggtga cggtttcttc agttctgcct 1021 tccaggcccg gctttcaggg aacaacctgc acaatgcttc catccctgaa cacctggcct 1081 acggctctgt gatcactgtg aagaacctcc ggatggccat cggctatctg cactcccaca 1141 ggcacctcta ccccgagggc attggtgccc gtcagcagca ggtcaccacc tatttgcaca 1201 aggactacaa caacctgtgg attatcaaga aacataacac aaactcagat cccctagacc 1261 cttccttccc agtggagttt gtaagacatg gagacattat tcgactagaa cacaaagaaa 1321 cttcccggaa cttgcacagt cactatcatg aggcccccat gacccggaag cactatcagg 1381 tcaccggcta tggcataaat ggaacagggg actcaaatga tttctggcgg attgaggtcg 1441 taaacagaaa atttggaaac cggatcaaag tgctgagaag tcgaattcgc ttcatccatt 1501 tggtcacagg ttgtgtcctg ggctcctcgg gaaaggttct gcccaagtgg ggctgggagc 1561 agttggaagt tacttgcacc ccatacctga aagaaaccct caactccatc tggaatgtgg 1621 aggaccatat caatcccaag ttgccaaaca tcagcctgga tgtgctacag cccagttttc 1681 ctgagatctt gctggaatcc cacatggtca tgatccgggg gaacagtggc ctcaaaccca 1741 aggacaatga gttcacgtcc aaaccctggc actggcctat caactatcag ggcctacgct 1801 tctcaggggt caatgacaca gatttccgag tctatctgct tggcaacccg gtggtttggt 1861 ggctGAATCT GTTGAGCATC GCCCTCTACC TCCTCTCAGG GAGCATCATT GCTGTAGCCA 1921 TGCAGAGAGG GGCACGGCTG CCAGCGGAGG TTGCAGGGTT GTCCCAGGTC CTGCTGCGAG 1981 GAGGCGGCCA GGTCCTGCTC GGCTGGACAC TCCATTACTT CCCGTTTTTC CTGATGGGCC 2041 GGGTCCTCTA CTTCCACCAC TACTTCCCAG CCATGCTCTT CTCAAGCATG TTGACAGGCA 2101 TTCTGTGGGA CACCCTCCTG CGGCTCTGTG CCTGGGGCTT GGCCTCATGG CCCCTGGCGA 2161 GGGGCATACA TGTGGCGGGA ATCCTGAGCC TGCTCCTGGG AACTGCCTAC AGCTTCTACC 2221 TCTTCCACCC TCTGGCTTAC GGGATGGTTG GTCCCCTGGC CCAGGACCCC CAAAGTCCAA 2281 TGGCAGGACT AAGGTGGCTG GACTCATGGG ACTTTTGCCC AACTTTCTTG TACAAAGTGG 2341 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2401 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2461 AGCCCCATGG AAAATAACTT CGGTTACGCG TTAAGTCgac aatcaacctc tggattacaa 2521 aatttgtgaa agatt