Construct: ORF TRCN0000481179
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003553.1_s317c1
- Derived from:
- ccsbBroadEn_16103
- DNA Barcode:
- CACCTTCATTAGAAACGGGCCTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLC3 (147700)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481179
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 147700 | KLC3 | kinesin light chain 3 | NM_177417.3 | 36.1% | 32.7% | (many diffs) |
| 2 | human | 147700 | KLC3 | kinesin light chain 3 | XM_024451371.1 | 32.8% | 29.8% | (many diffs) |
| 3 | human | 147700 | KLC3 | kinesin light chain 3 | XM_024451370.1 | 28% | 25.4% | (many diffs) |
| 4 | human | 147700 | KLC3 | kinesin light chain 3 | XM_024451369.1 | 26% | 23.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 654
- ORF length:
- 588
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgtgcaggta gcggctcctg gaagtgcagg gctgggccca gagcgcctga 121 gccctgagga gctggtgcgg cagacgcggc aagtggtcca ggggctggag gcgctgcggg 181 cagagcacca tggcctggct gggcacctgg cggaggccct ggcgggacag ggcccggcag 241 ccggcttgga gatgctggag gaaaagcagc aggtggtgag ccactcgctg gaggCCATCG 301 AGCTGGGGCT GGGCGAGGCC CAGGTGCTGC TGGCCCTGTC GGCACATGTG GGTGCACTGG 361 AGGCAGAGAA GCAGCGGCTG CGCTCGCAGG CCCGGCGGCT GGCCCAGGAG AACGTGTGGC 421 TGCGGGAGGA ACTGGAGGAG ACGCAGCGGC GGCTTCGGGC CAGCGAGGAG TCCGTGGCCC 481 AGCTGGAGGA GGAGAAGCGC CACCTGGAGT TCCTGGGGCA GCTGCGACAG TACGACCCAC 541 CGGCGGAGAG CCAGGTGCCA CGGGCAGGGC GAGGCGGGGG GTGCTGGGCC CTTCATAGAG 601 CCCCACAGTC CCCCAGACCC TCCTTAGAAT CCCACAGTCC CCAAGATCCT CCTTACCCAA 661 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 721 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 781 TATCTTGTGG AAAGGACGAC ACCTTCATTA GAAACGGGCC TCCACGCGTT AAGTCgacaa 841 tcaacctctg gattacaaaa tttgtgaaag att