Transcript: Human XM_024451370.1

PREDICTED: Homo sapiens kinesin light chain 3 (KLC3), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
KLC3 (147700)
Length:
2076
CDS:
103..2061

Additional Resources:

NCBI RefSeq record:
XM_024451370.1
NBCI Gene record:
KLC3 (147700)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024451370.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000425994 CGCTTTGGAGATCCGAGAGAA pLKO_005 1050 CDS 100% 4.950 6.930 N KLC3 n/a
2 TRCN0000180333 CTCCAAGATCCGTGAGTCTAT pLKO.1 1398 CDS 100% 4.950 6.930 N KLC3 n/a
3 TRCN0000183390 GAAACAGAACAAGTATCAACA pLKO.1 1260 CDS 100% 4.950 3.465 N KLC3 n/a
4 TRCN0000181188 CAACAACTTGGCTGTCCTCTA pLKO.1 984 CDS 100% 4.050 2.835 N KLC3 n/a
5 TRCN0000422487 GCATAACCTCGTGATCCAGTA pLKO_005 732 CDS 100% 4.050 2.835 N KLC3 n/a
6 TRCN0000178835 CAAGTACAAAGAAGCCACAGA pLKO.1 891 CDS 100% 2.640 1.848 N KLC3 n/a
7 TRCN0000180655 CCATGTCACTCAACACACTGA pLKO.1 1493 CDS 100% 2.640 1.848 N KLC3 n/a
8 TRCN0000149071 GAACAAGTATCAACAAGCGGA pLKO.1 1266 CDS 100% 0.660 0.462 N KLC3 n/a
9 TRCN0000417326 CAACGTGGCCAAGACCAAGAA pLKO_005 1218 CDS 100% 4.950 2.970 N KLC3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024451370.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09649 pDONR223 100% 77.2% 73% None 94C>A;1441_1708del;1781_1956del n/a
2 ccsbBroad304_09649 pLX_304 0% 77.2% 73% V5 94C>A;1441_1708del;1781_1956del n/a
3 TRCN0000472064 CCGCACGATGCGTTCACTTAATAT pLX_317 24.5% 77.2% 73% V5 94C>A;1441_1708del;1781_1956del n/a
4 ccsbBroadEn_13241 pDONR223 100% 77.1% 73% None 487_489delCAG;1441_1708del;1781_1956del n/a
5 ccsbBroad304_13241 pLX_304 0% 77.1% 73% V5 487_489delCAG;1441_1708del;1781_1956del n/a
6 TRCN0000467515 TAATACGATGCACAGACCATATCG pLX_317 21% 77.1% 73% V5 487_489delCAG;1441_1708del;1781_1956del n/a
7 ccsbBroadEn_16103 pDONR223 0% 28% 25.4% None (many diffs) n/a
8 ccsbBroad304_16103 pLX_304 0% 28% 25.4% V5 (many diffs) n/a
9 TRCN0000481179 CACCTTCATTAGAAACGGGCCTCC pLX_317 60.8% 28% 25.4% V5 (many diffs) n/a
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