Construct: ORF TRCN0000481199
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009325.1_s317c1
- Derived from:
- ccsbBroadEn_07586
- DNA Barcode:
- TCTAAGTCCGCGAATGTAATAGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- B3GALT5 (10317)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481199
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10317 | B3GALT5 | beta-1,3-galactosyltransfer... | NM_001278650.1 | 99.6% | 99.6% | 254T>C;318G>A;576G>A |
| 2 | human | 10317 | B3GALT5 | beta-1,3-galactosyltransfer... | NM_001356336.2 | 99.6% | 99.6% | 254T>C;318G>A;576G>A |
| 3 | human | 10317 | B3GALT5 | beta-1,3-galactosyltransfer... | NM_001356338.2 | 99.6% | 99.6% | 254T>C;318G>A;576G>A |
| 4 | human | 10317 | B3GALT5 | beta-1,3-galactosyltransfer... | NM_001356339.2 | 99.6% | 99.6% | 254T>C;318G>A;576G>A |
| 5 | human | 10317 | B3GALT5 | beta-1,3-galactosyltransfer... | NM_006057.3 | 99.6% | 99.6% | 254T>C;318G>A;576G>A |
| 6 | human | 10317 | B3GALT5 | beta-1,3-galactosyltransfer... | NM_033170.3 | 99.6% | 99.6% | 254T>C;318G>A;576G>A |
| 7 | human | 10317 | B3GALT5 | beta-1,3-galactosyltransfer... | NM_033171.3 | 99.6% | 99.6% | 254T>C;318G>A;576G>A |
| 8 | human | 10317 | B3GALT5 | beta-1,3-galactosyltransfer... | NM_033172.3 | 98.4% | 98.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 999
- ORF length:
- 930
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctttcccg aagatgagat tgatgtatat ttgccttctg gttctggggg 121 ctctttgttt gtattttagc atgtacagtc taaatccttt caaagaacag tcctttgttt 181 acaagaaaga cgggaacttc cttaagctcc cagatacaga ctgcaggcag acacctccct 241 tcctcgtcct gctggtgacc tcatcccaca aacagttggc tgagcgcatg gccatccggc 301 agacgtgggg gaaagagagg acggtgaagg gaaagcagct gaagacattc ttcctcctgg 361 ggaccaccag cagtgcagcg gaaacaaaag aggtggacca ggagagccag cgacacgggg 421 acattatcca gaaggatttc ctagacgtct attacaatct gaccctgaag accatgatgg 481 gcatagaatg ggtccatcgc ttttgtcctc aggcggcgtt tgtgatgaaa acagacTCAG 541 ACATGTTCAT CAATGTTGAC TATCTGACTG AACTGCTTCT GAAGAAAAAC AGAACAACCA 601 GGTTTTTCAC TGGCTTCTTG AAACTCAATG AGTTTCCCAT CAGACAGCCA TTCAGCAAGT 661 GGTTTGTCAG TAAATCTGAA TATCCGTGGG ACAGGTACCC ACCATTCTGC TCCGGCACCG 721 GCTACGTGTT TTCTGGCGAC GTGGCGAGTC AGGTGTACAA TGTCTCCAAG AGCGTCCCAT 781 ACATTAAACT GGAAGACGTG TTTGTGGGGC TCTGCCTCGA AAGGCTGAAC ATCAGATTGG 841 AGGAGCTCCA CTCCCAGCCG ACCTTTTTTC CAGGGGGCTT ACGCTTCTCC GTATGCCTCT 901 TCAGGAGGAT CGTGGCCTGC CACTTCATCA AGCCTCGGAC TCTCTTGGAC TACTGGCAGG 961 CTCTAGAGAA TTCCCGGGGG GAAGATTGTC CGCCTGTCTT GCCAACTTTC TTGTACAAAG 1021 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1081 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1141 ACGATCTAAG TCCGCGAATG TAATAGGTAC GCGTTAAGTC gacaatcaac ctctggatta 1201 caaaatttgt gaaagatt