Construct: ORF TRCN0000481199

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009325.1_s317c1
Derived from:
ccsbBroadEn_07586
DNA Barcode:
TCTAAGTCCGCGAATGTAATAGGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
B3GALT5 (10317)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481199
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10317 B3GALT5 beta-1,3-galactosyltransfer... NM_001278650.1 99.6% 99.6% 254T>C;318G>A;576G>A
2 human 10317 B3GALT5 beta-1,3-galactosyltransfer... NM_001356336.2 99.6% 99.6% 254T>C;318G>A;576G>A
3 human 10317 B3GALT5 beta-1,3-galactosyltransfer... NM_001356338.2 99.6% 99.6% 254T>C;318G>A;576G>A
4 human 10317 B3GALT5 beta-1,3-galactosyltransfer... NM_001356339.2 99.6% 99.6% 254T>C;318G>A;576G>A
5 human 10317 B3GALT5 beta-1,3-galactosyltransfer... NM_006057.3 99.6% 99.6% 254T>C;318G>A;576G>A
6 human 10317 B3GALT5 beta-1,3-galactosyltransfer... NM_033170.3 99.6% 99.6% 254T>C;318G>A;576G>A
7 human 10317 B3GALT5 beta-1,3-galactosyltransfer... NM_033171.3 99.6% 99.6% 254T>C;318G>A;576G>A
8 human 10317 B3GALT5 beta-1,3-galactosyltransfer... NM_033172.3 98.4% 98.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
999
ORF length:
930
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggctttcccg aagatgagat tgatgtatat ttgccttctg gttctggggg
121ctctttgttt gtattttagc atgtacagtc taaatccttt caaagaacag tcctttgttt
181acaagaaaga cgggaacttc cttaagctcc cagatacaga ctgcaggcag acacctccct
241tcctcgtcct gctggtgacc tcatcccaca aacagttggc tgagcgcatg gccatccggc
301agacgtgggg gaaagagagg acggtgaagg gaaagcagct gaagacattc ttcctcctgg
361ggaccaccag cagtgcagcg gaaacaaaag aggtggacca ggagagccag cgacacgggg
421acattatcca gaaggatttc ctagacgtct attacaatct gaccctgaag accatgatgg
481gcatagaatg ggtccatcgc ttttgtcctc aggcggcgtt tgtgatgaaa acagacTCAG
541ACATGTTCAT CAATGTTGAC TATCTGACTG AACTGCTTCT GAAGAAAAAC AGAACAACCA
601GGTTTTTCAC TGGCTTCTTG AAACTCAATG AGTTTCCCAT CAGACAGCCA TTCAGCAAGT
661GGTTTGTCAG TAAATCTGAA TATCCGTGGG ACAGGTACCC ACCATTCTGC TCCGGCACCG
721GCTACGTGTT TTCTGGCGAC GTGGCGAGTC AGGTGTACAA TGTCTCCAAG AGCGTCCCAT
781ACATTAAACT GGAAGACGTG TTTGTGGGGC TCTGCCTCGA AAGGCTGAAC ATCAGATTGG
841AGGAGCTCCA CTCCCAGCCG ACCTTTTTTC CAGGGGGCTT ACGCTTCTCC GTATGCCTCT
901TCAGGAGGAT CGTGGCCTGC CACTTCATCA AGCCTCGGAC TCTCTTGGAC TACTGGCAGG
961CTCTAGAGAA TTCCCGGGGG GAAGATTGTC CGCCTGTCTT GCCAACTTTC TTGTACAAAG
1021TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
1081GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
1141ACGATCTAAG TCCGCGAATG TAATAGGTAC GCGTTAAGTC gacaatcaac ctctggatta
1201caaaatttgt gaaagatt

Download FASTA (ORF) (Full)