Construct: ORF TRCN0000481204
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007397.1_s317c1
- Derived from:
- ccsbBroadEn_09555
- DNA Barcode:
- ACAGTGGATGACAATGTCTGTACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WDR36 (134430)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481204
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 134430 | WDR36 | WD repeat domain 36 | NM_139281.2 | 99.9% | 100% | 2142C>G |
| 2 | mouse | 225348 | Wdr36 | WD repeat domain 36 | NM_001110015.1 | 82% | 83.7% | (many diffs) |
| 3 | mouse | 225348 | Wdr36 | WD repeat domain 36 | NM_001110016.1 | 80.5% | 81.7% | (many diffs) |
| 4 | mouse | 225348 | Wdr36 | WD repeat domain 36 | XM_006525847.3 | 80.4% | 81.7% | (many diffs) |
| 5 | mouse | 225348 | Wdr36 | WD repeat domain 36 | NM_144863.4 | 54.9% | 55% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2922
- ORF length:
- 2853
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtgttgcact gagggcagtc tccggaaacg cgattcgcag cgggcgccgg 121 aagcggtgtt gtgtctgcag ctctggcaga ggactgttcc actagacacg ctgaagggac 181 tgggtacgtg ttttccttca ggaccagagc tgagaggagc tgggatcgcg gcggcaatgg 241 aacgggcctc agaaaggcgc acggccagcg cgctttttgc ggggttccgg gccttgggac 301 ttttcagcaa cgacattcca cacgtggtgc ggttcagcgc gctcaagcgc cggttctatg 361 taacaacctg cgtgggcaag agtttccaca cctatgacgt tcagaaactt agtctggttg 421 cagtaagtaa ttctgttcca caggatatct gctgtatggc agctgatggc agattagtct 481 ttgctgctta tggaaatgtt ttctctgcat ttgcccgtaa taaagagata gtacatacct 541 ttaagggtca taaggcagaa atccatttct tgcaaccctt tggagaccac attatctctg 601 ttgatactga tggcattctt attatttggc acatatattc agaagaagaa tacctgcagt 661 tgacttttga taaatcagta tttaaaattt ctgcaatttt gcatccaagt acctacttga 721 ataaaatact tctgggcagt gaacaaggaa gcctgcagtt gtggaatgta aaatccaata 781 aacttctata tacatttcca ggatggaaag ttggagtgac agctcttcag caggcaccag 841 ccgtggatgt tgttgctatt ggtcttatgt caggtcaagt tatcattcac aacattaaat 901 ttaatgaaac attaatgaag tttcgtcaag actggggacc cattacttca atttcatttc 961 gcacagatgg tcatccagta atggcagctg gaagcccatg tggccatatt ggactctggg 1021 atctagaaga caaaaaatta atcaaccaaa tgagaaatgc acactctaca gcaattgccg 1081 gactgacatt tctccataga gagccacttc ttgtcacaaa tggcgctgac aatgctctta 1141 ggatatggat atttgatggt cctacaggtg aaggccgact tttgagattc agaatgggtc 1201 atagtgctcc tcttaccaat atcagatatt atggacagaa tggacagcag attctaagtg 1261 caagtcaaga tggaactctt cagtcatttt ccacggtaca tgaaaaattc aataagagct 1321 tgggacatgg attaataaat aaaaagagag ttaaacgtaa aggacttcag aataccatgt 1381 cagtgagact tccacccatc acaaagtttg cagcagagga agctcgtgaa agtgactggg 1441 atggtatcat tgcttgccat caaggtaagc tatcttgctc aacctggaat tatcagaaat 1501 ctacaatagg cgcttacttt ctcaagccaa aagagttgaa gaaagatgac ataactgcaa 1561 cagcagtgga tataacttct tgtggaaact ttgctgtaat tggcctctca tcaggaactg 1621 tagatgtata taacatgcag tctggcatac atcgaggaag ttttggcaag gatcaagctc 1681 acaagggatc tgttagaggt gtcgcagtgg atggattaaa ccagttgaca gttacaactg 1741 gtagtgaagg attactcaaa ttctggaact ttaaaaacaa aattttaatc cattctgtga 1801 gcctcagttc atctccaaat atcatgttgc tacataggga cagtggcatt ctgggactcg 1861 ccttggatga cttctccatt agtgttctgg acatagaaac taggaagatt gtcagagagt 1921 tttctggaca ccaaggccaa ataaatgaca tggcttttag tcctgatggt cgttggttaa 1981 taagtgctgc gatggattgc tctattagga cttgggacct tccttctggg tgccttatag 2041 actgcttttt gttggactcg gctcctctca atgtttctat gtctcctact ggagactttc 2101 tggcaacttc ccatgtggac caccttggaa tttatctatg gtccaatatt tccctgtatt 2161 cagttgtttc attacggcca cttcctgcag attatgtccc ttcaatagtg atgcttcctg 2221 gtacttgtca aacccaagat gtagaagtat cagaagaaac agtagaacca agtgatgaat 2281 tgatagaata tgattcgcca gaacagttga atgagcaatt ggtgactctt tcacttcttc 2341 ctgaatcacg atggaaaaac cttcttaacc ttgatgttat taagaaaaag aataaaccaa 2401 aggaaccacc caaagtaccc aaatcagcac catttttcat tccaacaatt cctggccttg 2461 tacccagata tgctgcacct gaacaaaata atgatcccca gcagtctaaa gtggtaaatc 2521 ttggagtttt ggctcaaaaa tcagatttct gcttGAAACT TGAAGAAGGA CTGGTAAATA 2581 ATAAGTATGA CACTGCTCTC AACCTTCTGA AAGAATCAGG CCCATCAGGA ATTGAAACAG 2641 AGCTGCGAAG CTTGTCTCCT GATTGTGGTG GGTCCATAGA AGTTATGCAG AGCTTCTTGA 2701 AAATGATTGG GATGATGCTG GACAGAAAGC GTGATTTTGA GTTAGCCCAG GCATACCTTG 2761 CATTGTTTCT AAAGTTACAC CTTAAAATGC TTCCTTCAGA GCCAGTACTC CTAGAAGAAA 2821 TAACAAATTT GTCATCCCAG GTGGAAGAAA ACTGGACCCA TTTGCAATCA CTCTTCAATC 2881 AAAGCATGTG TATTTTAAAT TATCTCAAAA GTGCTTTGTT GTTGCCAACT TTCTTGTACA 2941 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 3001 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 3061 AGGACGAACA GTGGATGACA ATGTCTGTAC GACGCGTTAA GTCgacaatc aacctctgga 3121 ttacaaaatt tgtgaaagat t