Construct: ORF TRCN0000481211
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006118.1_s317c1
- Derived from:
- ccsbBroadEn_07817
- DNA Barcode:
- ATCTCATCCTGATGGAGTCTTGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MMRN1 (22915)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481211
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22915 | MMRN1 | multimerin 1 | NM_001371403.1 | 99.9% | 99.9% | 2891C>G |
| 2 | human | 22915 | MMRN1 | multimerin 1 | NM_007351.3 | 99.9% | 99.9% | 2891C>G |
| 3 | human | 22915 | MMRN1 | multimerin 1 | XM_005262856.3 | 97.1% | 97% | 848_849ins105;2786C>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3750
- ORF length:
- 3684
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gggggcaaga ttatttgtcc ttctttctag tttatggagt gggggcattg 121 ggcttaacaa cagtaagcat tcttggacta tacctgagga tgggaactct cagaagacta 181 tgccttctgc ttcagttcct ccaaataaaa tacaaagttt gcaaatactg ccaaccactc 241 gggtcatgtc ggcggagata gctacaactc cagaggcaag aacttctgaa gacagtcttc 301 ttaaatcaac actgcctccc tcagaaacaa gtgcacctgc tgagggtgtg agaaatcaaa 361 ctctcacatc cacagagaaa gcagaaggag tggtcaagtt acagaatctt accctcccaa 421 ccaacgctag catcaagttc aatcctggag cagaatcagt ggtcctttcc aattctacac 481 tgaaatttct tcagagcttt gccagaaagt caaatgaaca agcaacttct ctaaacacag 541 ttggaggcac tggaggcatt ggaggcgttg gaggcactgg aggcgtggga aatcgagccc 601 cacgggaaac atacctcagc cggggtgaca gcagttccag ccaaagaact gactaccaaa 661 aatcaaattt cgaaacaact agaggaaaga attggtgtgc ttatgtacat accaggttat 721 ctcccacagt gatattggac aaccaggtca cttatgtccc aggtgggaaa ggaccttgtg 781 gctggaccgg tggatcctgt cctcagagat ctcagaagat atccaatcct gtctatagga 841 tgcaacataa aattgtcacc tcattggatt ggaggtgctg tcctggatac agtgggccga 901 aatgtcaact aagagcccag gaacagcaaa gtttgataca caccaaccag gctgaaagtc 961 atacagctgt tggcagagga gtagctgagc agcagcagca gcaaggctgt ggtgacccag 1021 aagtgatgca aaaaatgact gatcaggtga actaccaggc aatgaaactg actcttctgc 1081 agaagaagat tgacaatatt tctttgactg tgaatgatgt aaggaacact tactcctccc 1141 tagaaggaaa agtcagcgaa gataaaagca gagaatttca atctcttcta aaaggtctaa 1201 aatccaaaag cattaatgta ctgataagag acatagtaag agaacaattt aaaatttttc 1261 aaaatgacat gcaagagact gtagcacagc tcttcaagac tgtatcaagt ctatcagagg 1321 acctcgaaag caccaggcaa ataattcaaa aagttaatga atctgtggtt tcaatagcag 1381 cccagcaaaa gtttgttttg gtgcaagaga atcggcccac tttgactgat atagtggaac 1441 taaggaatca cattgtgaat gtaaggcaag aaatgactct tacatgtgag aagcctatta 1501 aagaactaga agtaaagcag actcatttag aaggtgctct agaacaggaa cactcaagaa 1561 gcattctgta ttatgaatcc ctcaataaaa ctctttctaa attgaaggaa gtacatgagc 1621 agcttttatc aactgaacag gtatcagacc agaagaatgc tccagctgct gagtcagtta 1681 gcaataatgt cactgagtac atgtctactt tacatgaaaa tataaagaag cagagtttga 1741 tgatgctgca aatgtttgaa gatttgcaca ttcaagaaag caagattaac aatctcaccg 1801 tctctttgga gatggagaaa gagtctctca gaggtgaatg tgaagacatg ttatccaaat 1861 gcagaaatga ttttaaattt caacttaagg acacagaaga gaatttacat gtgttaaatc 1921 aaacattggc tgaagttctc tttccaatgg acaataagat ggacaaaatg agtgagcaac 1981 taaatgattt gacttatgat atggagatcc ttcaaccctt gcttgagcag ggagcatcac 2041 tcagacagac aatgacatat gaacaaccaa aggaagcaat agtgataagg aaaaagatag 2101 aaaatctgac tagtgctgtc aatagtctaa attttattat caaagaactt acaaaaagac 2161 acaacttact tagaaatgaa gtacagggtc gtgatgatgc cttagaaaga cgtatcaatg 2221 aatatgcctt agaaatggaa gatggcctca ataagacaat gactattata aataatgcta 2281 ttgatttcat tcaagataac tatgccctaa aagagacttt aagtactatt aaggataata 2341 gtgagatcca tcataaatgt acctccgata tggaaactat tttgacattt attcctcagt 2401 tccaccgtct gaatgattct attcagactt tggtcaatga caatcagaga tataactttg 2461 ttttgcaagt cgccaagacc cttgcaggta ttcccagaga tgagaaacta aatcagtcca 2521 acttccaaaa gatgtatcaa atgttcaatg aaaccacttc ccaagtgaga aaataccagc 2581 aaaatatgag tcatttggaa gaaaaactac tcttaactac caagatttcc aaaaattttg 2641 agactcggtt gcaagacatt gagtctaaag ttacccagac gctcatacct tattatattt 2701 cagttaaaaa aggcagtgta gttacaaatg agagagatca ggctcttcaa ctgcaagtat 2761 taaattccag atttaaggcg ttggaagcaa aatctatcca tctttcaatt aacttctttt 2821 cgcttaacaa aactctccac gaagttttaa caatgtgtca caatgcttct acaagtgtgt 2881 cagaactgaa tgctaccatc cctaagtgga taaaacattc cctgccagat attcaacttc 2941 ttcagaaagg tctaagagaa tttgtggaac caataattca aataaaaact caagctgccc 3001 tatctaattt aacttgttgt atagatcgat cgttgcctgg tagtctggca aatgttgtca 3061 agtctcagaa gcaagtaaaa tcattgccaa agaaaattaa cgcacttaag aaaccaacgg 3121 taaatcttac cacagtcctg ataggccgga ctcaaagaaa cacggacaac ataatatatc 3181 ctgaggagta ttcaagctgt agtcggcatc cgtgccaaaa tgggggcacg tgcataaatg 3241 gaagaactag ctttacctgt gcctgcagac atccttttac tggtgacaac tgcactatca 3301 agcttgtgga agaaaatgct ttagctccag atttttccaa aggatcttac agatatgcac 3361 ccatggtggC ATTTTTTGCA TCTCATACGT ATGGAATGAC TATACCTGGT CCTATCCTGT 3421 TTAATAACTT GGATGTCAAT TATGGAGCTT CATATACCCC AAGAACTGGA AAATTTAGAA 3481 TTCCGTATCT TGGAGTATAT GTTTTCAAGT ACACCATCGA GTCATTTAGT GCTCATATTT 3541 CTGGATTTTT AGTGGTTGAT GGAATAGACA AGCTTGCATT TGAGTCTGAA AATATTAACA 3601 GTGAAATACA CTGTGATAGG GTTTTAACTG GGGATGCCTT ATTAGAATTA AATTATGGGC 3661 AGGAAGTCTG GTTACGACTT GCAAAAGGAA CAATTCCAGC CAAGTTTCCC CCTGTTACTA 3721 CATTTAGTGG CTATTTATTA TATCGTACAT ACCCAACTTT CTTGTACAAA GTGGTTGATA 3781 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 3841 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAATCTC 3901 ATCCTGATGG AGTCTTGTAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 3961 tgaaagatt