Construct: ORF TRCN0000481214
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000872.1_s317c1
- Derived from:
- ccsbBroadEn_04534
- DNA Barcode:
- CCCCCAATCGTGCTCCTTCACCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- JPT2 (90861)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481214
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 90861 | JPT2 | Jupiter microtubule associa... | NM_144570.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 636
- ORF length:
- 570
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ccaggtcccg gatagcgagg gcggccgcgc cggctccagg gccatgaagc 121 ccccaggagg agaatcgagc aatctttttg gaagtccaga agaagctact ccttccagca 181 ggccTAATAG GATGGCATCT AATATTTTTG GACCAACAGA AGAACCTCAG AACATACCCA 241 AGAGGACAAA TCCCCCAGGG GGTAAAGGAA GTGGTATCTT TGACGAATCA ACCCCCGTGC 301 AGACTCGACA GCACCTGAAC CCACCTGGAG GGAAGACCAG CGACATTTTT GGGTCTCCGG 361 TCACTGCCAC TTCACGCTTG GCACACCCAA ACAAACCCAA GGATCATGTT TTCTTATGTG 421 AAGGAGAAGA ACCAAAATCG GATCTTAAAG CTGCAAGGAG CATCCCGGCT GGAGCAGAGC 481 CAGGTGAGAA AGGCAGCGCC AGAAAAGCAG GCCCCGCCAA GGAGCAGGAG CCCATGCCCA 541 CAGTCGACAG CCATGAGCCC CGGCTGGGGC CGCGGCCTCG CTCTCACAAC AAGGTCCTGA 601 ACCCACCGGG AGGCAAATCC AGCATCTCCT TCTACTACCC AACTTTCTTG TACAAAGTGG 661 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 721 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 781 ACCCCCAATC GTGCTCCTTC ACCTGACGCG TTAAGTCgac aatcaacctc tggattacaa 841 aatttgtgaa agatt