Construct: ORF TRCN0000481227
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010136.1_s317c1
- Derived from:
- ccsbBroadEn_11914
- DNA Barcode:
- GAGTAGGTAGTCAGTGATGATAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRICKLE4 (29964)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481227
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29964 | PRICKLE4 | prickle planar cell polarit... | NM_013397.6 | 88.6% | 88.5% | 1_124del;127_128insTCTC;861_862insCTT |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1104
- ORF length:
- 1035
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtctcctcag ggtcctgcag ttctgagctt gggttccctt tgcctggaca 121 ccaaccaagc ccccaactgg actggacttc agaccctcct gcagcaactc cctccgcagg 181 acattgatga gcgctactgc ctggcccttg gggaggagga gcgggccgag ctgcagctct 241 tctgtgccag gcggaagcag gaagccctgg gacagggggt agcccgcctg gtacttccca 301 agcttgaagg acacacctgt gagaagtgta gggagctgct gaagccaggg gagtacggag 361 tgtttgcagc ccgggcaggg gaacagcgct gctggcacca gccttgcttt gcctgccagg 421 cctgtggcca ggccctgata aacctcatct acttctacca tgatggacaa ctctactgcg 481 gccgtcatca tgcagagttg ctgcgcccgc gctgcccggc ttgtgaccag ctgatcttct 541 cctggcgctg caccgaggcg gagggacagc gctggcatga gaaccacttc tgttgccagg 601 actgcgccgg gcctctgggc gggggacgtt atgccctgcc tgggggaagc ccctgctgcc 661 ccagctgctt cgagaaccgc tactcggatg caggctcgag ctgggccggg gCACTGGAAG 721 GGCAGGCATT CCTTGGGGAG ACTGGACTCG ACCGAACTGA AGGAAGGGAC CAAACCTCGG 781 TGAACTCTGC AACCCTCTCC CGAACACTCC TTCTCGCTGC TGCCGGCGGT TCCAGCCTGC 841 AAACTCAGAG GGGGCTGCCT GGATCCAGTC CCCAGCAGGA GAACCGACCT GGGGACAAAG 901 CGGAGGCACC CAAAGGGCAG GAGCAATGCC GCCTGGAGAC TATTCGTGAT CCCAAGGACA 961 CCCCTTTCTC CACCTGCTCC TCCTCCTCTG ACTCGGAACC TGAAGGATTT TTCTTAGGCG 1021 AGCGCCTTCC CCAGTCCTGG AAGACCCCCG GAAGCCTCCA AGCAGAGGAC AGCAACGCCT 1081 CTAAGACGCA CTGCACCATG TGCTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1141 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1201 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG AGTAGGTAGT 1261 CAGTGATGAT AATACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1321 att