Construct: ORF TRCN0000481237

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000298.1_s317c1
Derived from:
ccsbBroadEn_09334
DNA Barcode:
TTAACGTTGGTGCGGTGCTATATA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CAPN13 (92291)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481237
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 92291 CAPN13 calpain 13 NM_144575.3 99.9% 99.8% 838G>A;948G>A
2 human 92291 CAPN13 calpain 13 XM_011533159.3 99.9% 99.8% 838G>A;948G>A
3 human 92291 CAPN13 calpain 13 XM_011533160.2 99.9% 99.8% 838G>A;948G>A
4 human 92291 CAPN13 calpain 13 XM_011533161.3 99.9% 99.8% 838G>A;948G>A
5 human 92291 CAPN13 calpain 13 XM_017005264.1 99.9% 99.8% 838G>A;948G>A
6 human 92291 CAPN13 calpain 13 XM_017005266.2 99.3% 99.2% 838G>A;948G>A;1235_1236insGGCTGGCTCACA
7 human 92291 CAPN13 calpain 13 XR_939741.3 66% (many diffs)
8 human 92291 CAPN13 calpain 13 XM_011533163.3 63.7% 62.9% (many diffs)
9 human 92291 CAPN13 calpain 13 XR_939742.3 62.8% (many diffs)
10 human 92291 CAPN13 calpain 13 XR_939743.3 52.5% (many diffs)
11 human 92291 CAPN13 calpain 13 XR_001739074.1 49% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2076
ORF length:
2007
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggcgtattac caggagcctt cagtggagac ctccatcatc aagttcaaag
121accaggactt taccaccttg cgggatcact gcctgagcat gggccggacg tttaaggatg
181agacattccc tgcagcagat tcttccatag gccagaagct gctccaggaa aaacgcctct
241ccaatgtgat atggaagcgg ccacaggatc taccaggggg tcctcctcac ttcatcctgg
301atgatataag cagatttgac atccaacaag gaggcgcagc tgactgctgg ttcctggcag
361cactgggatc cttgactcag aacccacagt acaggcagaa gatcctgatg gtccaaagct
421tttcacacca gtatgctggc attttccgtt tccggttctg gcaatgtggc cagtgggtgg
481aagtggtgat tgatgaccgc ctacctgtcc agggagataa atgcctcttt gtgcgtcctc
541gccaccaaaa ccaagagttc tggccctgcc tgctggagaa ggcctatgcc aagctgctcg
601gatcctattc cgatctgcac tatggcttcc tcgaggatgc cctggtggac ctcacaggag
661gcgtgatcac caacatccat ctgcactctt cccctgtgga cctggtgaag gcagtgaaga
721cagcgaccaa ggcaggctcc ctgataacct gtgccactcc aagtgggcca acagatacag
781cacaggcgat ggagaatggg ctggtgagtc tccatgccta cactgtgact ggggctgagc
841agattcaata ccgaaggggc tgggaagaaa ttatctccct gtggaacccc tggggctggg
901gcgagaccga atggagaggg cgctggagtg atgggtctca ggagtgggag gaaacctgtg
961atccgcggaa aagccagcta cataagaaac gggaagatgg cgagttttgg atgtcatgtc
1021aagatttcca acagaaattc atcgccatgt ttatatgtag cgaaattcca attaccctgg
1081accatggaaa cacactccac gaaggatggt cccaaataat gtttaggaag caagtgattc
1141taggaaacac tgcaggagga cctcggaatg atgctcaatt caacttctct gtgcaagagc
1201caatggaagg caccaatgtt gtcgtgtgcg tcacagttgc tgtcacacca tcaaatttga
1261aagcagaaga tgcaaaattt ccactcgatt tccaagtgat tctggctggc tcacagcggt
1321tccgggagaa atttccaccc gtgttttttt cctcgttcag aaacactgtc caaagctcaa
1381ataataaatt ccgccgcaac ttcaccatga cttaccatct gagccctggg aactatgttg
1441tggttgcaca gacacggaga aaatcagcgg agttcttgct ccgaatcttc ctgaaaatgc
1501cagacagtga caggcacctg agcagccatt tcaacctcag aatgaaggga agcccttcag
1561aacatggctc ccaacaaagc attttcaaca gatatgctca gcagaggctg gacattgatg
1621ccacccagct tcagggcctt ctcaaccagg agcttctaac aggacctcca ggggacatgt
1681tctccTTAGA TGAGTGCCGC AGCTTGGTGG CTCTGATGGA ACTGAAAGTG AATGGGCGGC
1741TAGACCAAGA GGAGTTTGCG CGACTGTGGA AGCGCCTTGT TCACTACCAG CATGTTTTCC
1801AGAAGGTTCA GACAAGCCCT GGAGTCCTCC TGAGCTCGGA CTTGTGGAAG GCCATAGAGA
1861ATACAGACTT CCTCAGAGGG ATCTTCATCA GCCGTGAGCT GCTGCATCTG GTGACCCTCA
1921GGTACAGCGA CAGCGTCGGC AGGGTCAGCT TCCCCAGCCT GGTCTGCTTC CTGATGCGGC
1981TTGAAGCCAT GGCAAAGACC TTCCGCAACC TCTCTAAGGA TGGAAAAGGA CTCTACCTGA
2041CAGAAATGGA GTGGATGAGC CTGGTCATGT ACAACTTGCC AACTTTCTTG TACAAAGTGG
2101TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
2161CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
2221ATTAACGTTG GTGCGGTGCT ATATAACGCG TTAAGTCgac aatcaacctc tggattacaa
2281aatttgtgaa agatt

Download FASTA (ORF) (Full)