Construct: ORF TRCN0000481237
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000298.1_s317c1
- Derived from:
- ccsbBroadEn_09334
- DNA Barcode:
- TTAACGTTGGTGCGGTGCTATATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAPN13 (92291)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481237
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92291 | CAPN13 | calpain 13 | NM_144575.3 | 99.9% | 99.8% | 838G>A;948G>A |
| 2 | human | 92291 | CAPN13 | calpain 13 | XM_011533159.3 | 99.9% | 99.8% | 838G>A;948G>A |
| 3 | human | 92291 | CAPN13 | calpain 13 | XM_011533160.2 | 99.9% | 99.8% | 838G>A;948G>A |
| 4 | human | 92291 | CAPN13 | calpain 13 | XM_011533161.3 | 99.9% | 99.8% | 838G>A;948G>A |
| 5 | human | 92291 | CAPN13 | calpain 13 | XM_017005264.1 | 99.9% | 99.8% | 838G>A;948G>A |
| 6 | human | 92291 | CAPN13 | calpain 13 | XM_017005266.2 | 99.3% | 99.2% | 838G>A;948G>A;1235_1236insGGCTGGCTCACA |
| 7 | human | 92291 | CAPN13 | calpain 13 | XR_939741.3 | 66% | (many diffs) | |
| 8 | human | 92291 | CAPN13 | calpain 13 | XM_011533163.3 | 63.7% | 62.9% | (many diffs) |
| 9 | human | 92291 | CAPN13 | calpain 13 | XR_939742.3 | 62.8% | (many diffs) | |
| 10 | human | 92291 | CAPN13 | calpain 13 | XR_939743.3 | 52.5% | (many diffs) | |
| 11 | human | 92291 | CAPN13 | calpain 13 | XR_001739074.1 | 49% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2076
- ORF length:
- 2007
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgtattac caggagcctt cagtggagac ctccatcatc aagttcaaag 121 accaggactt taccaccttg cgggatcact gcctgagcat gggccggacg tttaaggatg 181 agacattccc tgcagcagat tcttccatag gccagaagct gctccaggaa aaacgcctct 241 ccaatgtgat atggaagcgg ccacaggatc taccaggggg tcctcctcac ttcatcctgg 301 atgatataag cagatttgac atccaacaag gaggcgcagc tgactgctgg ttcctggcag 361 cactgggatc cttgactcag aacccacagt acaggcagaa gatcctgatg gtccaaagct 421 tttcacacca gtatgctggc attttccgtt tccggttctg gcaatgtggc cagtgggtgg 481 aagtggtgat tgatgaccgc ctacctgtcc agggagataa atgcctcttt gtgcgtcctc 541 gccaccaaaa ccaagagttc tggccctgcc tgctggagaa ggcctatgcc aagctgctcg 601 gatcctattc cgatctgcac tatggcttcc tcgaggatgc cctggtggac ctcacaggag 661 gcgtgatcac caacatccat ctgcactctt cccctgtgga cctggtgaag gcagtgaaga 721 cagcgaccaa ggcaggctcc ctgataacct gtgccactcc aagtgggcca acagatacag 781 cacaggcgat ggagaatggg ctggtgagtc tccatgccta cactgtgact ggggctgagc 841 agattcaata ccgaaggggc tgggaagaaa ttatctccct gtggaacccc tggggctggg 901 gcgagaccga atggagaggg cgctggagtg atgggtctca ggagtgggag gaaacctgtg 961 atccgcggaa aagccagcta cataagaaac gggaagatgg cgagttttgg atgtcatgtc 1021 aagatttcca acagaaattc atcgccatgt ttatatgtag cgaaattcca attaccctgg 1081 accatggaaa cacactccac gaaggatggt cccaaataat gtttaggaag caagtgattc 1141 taggaaacac tgcaggagga cctcggaatg atgctcaatt caacttctct gtgcaagagc 1201 caatggaagg caccaatgtt gtcgtgtgcg tcacagttgc tgtcacacca tcaaatttga 1261 aagcagaaga tgcaaaattt ccactcgatt tccaagtgat tctggctggc tcacagcggt 1321 tccgggagaa atttccaccc gtgttttttt cctcgttcag aaacactgtc caaagctcaa 1381 ataataaatt ccgccgcaac ttcaccatga cttaccatct gagccctggg aactatgttg 1441 tggttgcaca gacacggaga aaatcagcgg agttcttgct ccgaatcttc ctgaaaatgc 1501 cagacagtga caggcacctg agcagccatt tcaacctcag aatgaaggga agcccttcag 1561 aacatggctc ccaacaaagc attttcaaca gatatgctca gcagaggctg gacattgatg 1621 ccacccagct tcagggcctt ctcaaccagg agcttctaac aggacctcca ggggacatgt 1681 tctccTTAGA TGAGTGCCGC AGCTTGGTGG CTCTGATGGA ACTGAAAGTG AATGGGCGGC 1741 TAGACCAAGA GGAGTTTGCG CGACTGTGGA AGCGCCTTGT TCACTACCAG CATGTTTTCC 1801 AGAAGGTTCA GACAAGCCCT GGAGTCCTCC TGAGCTCGGA CTTGTGGAAG GCCATAGAGA 1861 ATACAGACTT CCTCAGAGGG ATCTTCATCA GCCGTGAGCT GCTGCATCTG GTGACCCTCA 1921 GGTACAGCGA CAGCGTCGGC AGGGTCAGCT TCCCCAGCCT GGTCTGCTTC CTGATGCGGC 1981 TTGAAGCCAT GGCAAAGACC TTCCGCAACC TCTCTAAGGA TGGAAAAGGA CTCTACCTGA 2041 CAGAAATGGA GTGGATGAGC CTGGTCATGT ACAACTTGCC AACTTTCTTG TACAAAGTGG 2101 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2161 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2221 ATTAACGTTG GTGCGGTGCT ATATAACGCG TTAAGTCgac aatcaacctc tggattacaa 2281 aatttgtgaa agatt