Construct: ORF TRCN0000481245
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013155.1_s317c1
- Derived from:
- ccsbBroadEn_15198
- DNA Barcode:
- ATGTCGACGATTCAATCGATCGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZGPAT (84619)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481245
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84619 | ZGPAT | zinc finger CCCH-type and G... | NM_001083113.1 | 99.4% | 98.8% | (many diffs) |
| 2 | human | 84619 | ZGPAT | zinc finger CCCH-type and G... | NM_001195653.1 | 99.4% | 98.8% | (many diffs) |
| 3 | human | 84619 | ZGPAT | zinc finger CCCH-type and G... | NM_181485.2 | 99.4% | 98.8% | (many diffs) |
| 4 | human | 84619 | ZGPAT | zinc finger CCCH-type and G... | NM_001195654.1 | 97.7% | 97% | (many diffs) |
| 5 | human | 84619 | ZGPAT | zinc finger CCCH-type and G... | NM_032527.4 | 95.7% | 95.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1599
- ORF length:
- 1533
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cgaggagagc ctgggnccgc ccttgcagac ctaccgtgcg cagctgcagc 121 aggtggagct ggccttgggc gccggcctgg attcgtctga gcaggctgac ctgcgccagc 181 tgcaggggga cctgaaggag ctcatcgagc tcaccgaggc cagcctggtg tctgtcagga 241 agagcaggtt gttggccgcg ctggacgagg agagcctggg ccgccaggaa gatgctgagt 301 accaggcttt ccgggaggcc atcactgagg cggtggaggc accagcagcg gcccgtgggt 361 ccggatcaga gaccgttcct aaagcagagg cggggccaga atctgcggca ggtgggcagg 421 aggaggaaga gggagaggac gaggaagagc tgagtgggac aaaggtgagc gcgccctact 481 acagctcctg gggcactctg gagtatcaca acgccatggt ggtgggaacg gaagaggcgg 541 aggatggctc ggcgggtgtc cgtgtgcttt acctgtaccc cactcacaag tctctgaagc 601 cgtgcccgtt cttcctggag ggaaagtgcc gctttaagga gaactgcagg ttctcccatg 661 ggcaggtggt ctctctggat gagctgcgcc ccttccagga cccagacctg agctccctgc 721 aggccggctc tgcgtgtctg gccaagcacc aggatggcct ctggcacgca gcacgcatca 781 ccgatgtgga caacggctac tacacagtca agtttgactc gctgctgctg agggaggccg 841 tggtggaggg ggacggcatc ctgcccccac tgcgcacaga ggccacagag tccgactcag 901 acagcgacgg tacgggtgac tccagctatg ccagagtggt ggggtcagat gctgtggact 961 ctgggacctg cagctctgcc tttgctggct gggaggtgca cacgcgaggt ataggctcca 1021 gactcctcac caagatgggc tatgagtttg gcaagggttt gggccgacac gcggaaggcc 1081 gggtggagcc catccatgct gtggtgttgc ctcgagggaa gtcgctggac cagtgtgtgg 1141 agaccctgca gaagcagacc agggttggca aggctggcac caacaagCCC CCCAGGTGCC 1201 GGGGAAGAGG GGCCAGGCCT GGGGGCCGCC CAGCTCCTCG GAATGTGTTT GACTTCCTCA 1261 ATGAAAAGCT GCAAGGTCAG GCTCCTGGGG CCCTAGAAGC CGGGGCGGCC CCAGCGGGGA 1321 GGAGGAGCAA GGACATGTAC CATGCCAGCA AGAGTGCCAA GCGGGCCCTG AGCCTGCGGC 1381 TCTTCCAGAC TGAGGAGAAG ATCGAGCGAA CCCAGCGGGA CATCAGGAGC ATCCAGGAGG 1441 CTCTCGCCCG CAACGCTGGC CGGCATAGCG TGGCGTCAGC CCAGCTGCAG GAGAAGCTGG 1501 CAGGAGCCCA GCGCCAGCTG GGGCAGCTCC GGGCTCAGGA AGCCGGCCTG CAGCAGGAGC 1561 AGAGGAAGGC AGACACCCAC AAGAAGATGA CTGAGTTCTA CCCAACTTTC TTGTACAAAG 1621 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1681 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1741 ACGAATGTCG ACGATTCAAT CGATCGATAC GCGTTAAGTC gacaatcaac ctctggatta 1801 caaaatttgt gaaagatt