Construct: ORF TRCN0000481257
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002847.1_s317c1
- Derived from:
- ccsbBroadEn_05739
- DNA Barcode:
- CGTCCTTTACTCCTGCCCTTAGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM166B (730112)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481257
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 730112 | FAM166B | family with sequence simila... | NM_001099951.4 | 100% | 100% | |
| 2 | human | 730112 | FAM166B | family with sequence simila... | NM_001287238.2 | 82.1% | 67.8% | 493_524del;681_789del |
| 3 | human | 730112 | FAM166B | family with sequence simila... | NM_001164310.3 | 78.5% | 70.5% | 542_609del;717_825del |
| 4 | human | 730112 | FAM166B | family with sequence simila... | NM_001287239.2 | 70.5% | 60.5% | 542_563del;671_918del |
| 5 | human | 730112 | FAM166B | family with sequence simila... | XM_011518028.2 | 65.4% | 52% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 717
- ORF length:
- 648
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgtggcc agcaccttca taccagggct caaccctcag aaccctcatt 121 atatcccagg gtacactgga cactgcccac tacttcggtt cagcgtgggc cagacctatg 181 ggcaggtgac tggtcagcta cttcgaggcc ctcctggcct agcctggccc cctgtccacc 241 gcacacttct gcctcccatt cggcctccaa gatctcctga ggttcccagg gagagtctac 301 ctgtcaggcg tgggcaagaa aggctcagcT CCAGCATGAT CCCTGGGTAC ACAGGTTTTG 361 TACCCCGGGC ACAGTTCATC TTTGCCAAGA ACTGCAGCCA GGTCTGGGCC GAGGCTCTGA 421 GTGACTTTAC TCACTTGCAT GAAAAGCAAG GGAGTGAAGA GCTACCAAAG GAGGCCAAGG 481 GAAGAAAGGA CACAGAGAAG GACCAGGTGC CAGAGCCGGA GGGGCAGCTG GAGGAGCCGA 541 CACTGGAGGT GGTGGAACAA GCTTCTCCCT ACTCCATGGA TGACAGGGAC CCTCGGAAGT 601 TCTTCATGTC AGGCACTGCA GGAATTTGGG CAGAAGCACT CACCAGGCAG TGCCCAGGAC 661 CCCAAACATC TCCCCCCACT TCCCAGAACA TACCCTCAGA ACCTGGGTCT TTTACCTTGC 721 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 781 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 841 ATATATCTTG TGGAAAGGAC GACGTCCTTT ACTCCTGCCC TTAGATACGC GTTAAGTCga 901 caatcaacct ctggattaca aaatttgtga aagatt