Construct: ORF TRCN0000481264
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000696.1_s317c1
- Derived from:
- ccsbBroadEn_11360
- DNA Barcode:
- TGTCATCAGGATTTCCTCACTCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPIG (9360)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481264
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9360 | PPIG | peptidylprolyl isomerase G | XM_017005302.2 | 73.7% | 71.4% | (many diffs) |
| 2 | human | 9360 | PPIG | peptidylprolyl isomerase G | NM_004792.3 | 46.5% | 45.1% | (many diffs) |
| 3 | human | 9360 | PPIG | peptidylprolyl isomerase G | XM_005246966.2 | 46.5% | 45.1% | (many diffs) |
| 4 | human | 9360 | PPIG | peptidylprolyl isomerase G | XM_005246967.1 | 46.5% | 45.1% | (many diffs) |
| 5 | mouse | 228005 | Ppig | peptidyl-prolyl isomerase G... | NM_001081086.1 | 41.9% | 43.8% | (many diffs) |
| 6 | mouse | 228005 | Ppig | peptidyl-prolyl isomerase G... | XM_006499184.3 | 41.9% | 43.8% | (many diffs) |
| 7 | mouse | 228005 | Ppig | peptidyl-prolyl isomerase G... | XM_006499185.3 | 41.9% | 43.8% | (many diffs) |
| 8 | mouse | 228005 | Ppig | peptidyl-prolyl isomerase G... | XM_006499186.2 | 41.9% | 43.8% | (many diffs) |
| 9 | mouse | 228005 | Ppig | peptidyl-prolyl isomerase G... | XM_006499187.3 | 41.9% | 43.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1137
- ORF length:
- 1071
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg aataaaggtt caacgtcctc gatgtttttt tgacattgcc attaacaatc 121 aacctgctgg aagagttgtc tttgaattat tttctgatgt gtgccccaaa acatgcgaga 181 actttcgttg tctttgtaca ggtgaaaagg ggaccgggaa atcaactcag aaaccattac 241 attataagag ttgtctcttt cacagagttg tcaaggattt tatggttcaa ggtggtgact 301 tcagtgaagg aaatggacga ggaggggaat ctatctatgg aggatttttt gaagacgaga 361 gtttcgctgt taaacacaac aaagaatttc tcttgtcaat ggccaacaga gggaaggata 421 caaatggttc acagttcttc ataacaacga aaccaactcc tcatttagat gggcatcatg 481 ttgtttttgg acaagtaatc tctggtcaag aagttgtaag agagattgaa aaccagaaaa 541 cagatgcagc tagcaaaccg tttgcggagg tacggatact cagttgtgga gagctgattc 601 ccaaatctaa agttaagaaa gaagaaaaga aaaggcataa atcatcatca tcttcctcct 661 cctcatctag tgactcagat agctcaagtg attctcagTC CTCTTCTGAT TCCTCTGATT 721 CCGAAAGTGC TACTGAAGAG AAATCAAAGA AAAGAAAAAA GAAACATCGG AAAAATTCCC 781 GAAAACACAA GAAAGAAAAG AAAAAGCGAA AGAAAAGCAA GAAGAGTGCA TCTAGTGAGA 841 GTGAAGCTGA AAATCTTGAA GCACAACCCC AGTCTACTGT CCGTCCAGAA GAGATCCCTC 901 CTATACCTGA AAATAGATTC CTAATGAGAA AAAGTCCTCC TAAAGCTGAT GAGAAGGAAA 961 GGAAAAACAG AGAGAGAGAA AGGGAAAGAG AGTGTAATCC ACCTAACTCC CAGCCTGCTT 1021 CATACCAGAG ACGACTTTTA GTTACTAGAT CTGGCAGGAA AATTAAAGGA AGAGGACCAA 1081 GGGTAGGTGA TTCTTTCCCC AGAGATCTTC ACAATATTGC ATTTGTCTTC CTTAAATACC 1141 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1201 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1261 ATATATCTTG TGGAAAGGAC GATGTCATCA GGATTTCCTC ACTCTAACGC GTTAAGTCga 1321 caatcaacct ctggattaca aaatttgtga aagatt