Construct: ORF TRCN0000481284
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008438.1_s317c1
- Derived from:
- ccsbBroadEn_01334
- DNA Barcode:
- CGATCTCTAGACCCTGATGTGCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTGIS (5740)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481284
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5740 | PTGIS | prostaglandin I2 synthase | NM_000961.4 | 100% | 100% | |
| 2 | mouse | 19223 | Ptgis | prostaglandin I2 (prostacyc... | NM_008968.3 | 83.8% | 85.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1569
- ORF length:
- 1500
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcttgggcc gcgctcctcg gcctcctggc cgcactgttg ctgctgctgc 121 tactgagccg ccgccgcacg cggcgacctg gtgagcctcc cctggacctg ggcagcatcc 181 cctggttggg gtatgccttg gactttggaa aagatgctgc cagcttcctc acgaggatga 241 aggagaagca cggtgacatc tttactatac tggttggggg caggtatgtc accgttctcc 301 tggacccaca ctcctacgac gcggtggtgt gggagcctcg caccaggctc gacttccatg 361 cctatgccat cttcctcatg gagaggattt ttgatgtgca gcttccacat tacagcccca 421 gtgatgaaaa ggccaggatg aaactgactc ttctccacag agagctccag gcactcacag 481 aagccatgta taccaacctc catgcagtgc tgttgggcga tgctacagaa gcaggcagtg 541 gctggcacga gatgggtctc ctcgacttct cctacagctt cctgctcaga gccggctacc 601 tgactcttta cggaattgag gcgctgccac gcacccatga aagccaggcc caggaccgcg 661 tccactcagc tgatgtcttc cacacctttc gccagctcga ccggctgctc cccaaactgg 721 cccgtggctc cctgtcagtg ggggacaagg accacatgtg cagtgtcaaa agtcgcctgt 781 ggaagctgct atccccagcc aggctggcca ggcgggccca ccggagcaaa tggctggaga 841 gttacctgct gcacctggag gagatgggtg tgtcagagga gatgcaggca cgggccctgg 901 tgctgcagct gtgggccaca caggggaata tgggtcccgc tgccttctgg ctcctgctct 961 tccttctcaa gaatcctgaa gccctggctg ctgtccgcgg agagctcgag agtatccttt 1021 ggcaagcgga gcagcctgtc tcgcagacga ccactctccc acagaaggtt ctagacagca 1081 cacctgtgct tgatagcgtg ctgagtgaga gcctcaggct tacagctgcc cccttcatca 1141 cccgcgaggt tgtggtggac ctggccatgc ccatggcaga cgggcgagaa ttcaaccTGC 1201 GACGTGGTGA CCGCCTCCTC CTCTTCCCCT TCCTGAGCCC CCAGAGAGAC CCAGAAATCT 1261 ACACAGACCC AGAGGTATTT AAATACAACC GATTCCTGAA CCCTGACGGA TCAGAGAAGA 1321 AAGACTTTTA CAAGGATGGG AAACGGCTGA AGAATTACAA CATGCCCTGG GGGGCGGGGC 1381 ACAATCACTG CCTGGGGAGG AGTTATGCGG TCAACAGCAT CAAACAATTT GTGTTCCTTG 1441 TGCTGGTGCA CTTGGACTTG GAGCTGATCA ACGCAGATGT GGAGATCCCT GAGTTTGACC 1501 TCAGCAGGTA CGGCTTCGGT CTGATGCAGC CGGAACACGA CGTGCCCGTC CGCTACCGCA 1561 TCCGCCCATT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1621 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1681 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACGATCT CTAGACCCTG ATGTGCAAAC 1741 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt