Construct: ORF TRCN0000481305
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011346.1_s317c1
- Derived from:
- ccsbBroadEn_13738
- DNA Barcode:
- TCAATTTAGCCGGCGCCGGGTAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC728138 (728138)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481305
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 90231 | KIAA2013 | KIAA2013 | NM_138346.3 | 98.2% | 96.8% | (many diffs) |
| 2 | mouse | 77034 | 2510039O18Rik | RIKEN cDNA 2510039O18 gene | NM_029841.3 | 87.2% | 89.7% | (many diffs) |
| 3 | mouse | 77034 | 2510039O18Rik | RIKEN cDNA 2510039O18 gene | XM_006539279.3 | 79.8% | 82.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1968
- ORF length:
- 1899
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtggctacag caggggctca aggggctgcc gggactgctg tcgagcagct 121 gggcccgccg cctcctctgc ctgcttggcc tcctactgct gcttctgtgg tttggggggt 181 ccggcgcgca gcgggcggcg ggcggcctgc acctgctgcc ctggtcctgg ggtgagccgg 241 gcgccaccga gccgtctgcc tgcctggagg cggccacccg cgcctggcgc ggcctgcggg 301 agcgcggcga ggcggtaccg ctgggccctg gagtgccggc cctggtggcc aacggcttcc 361 tggccctgga cgtggctgcc aatcggctgt gggtgactcc cggggagcgg gagcccgccg 421 tggcgccgga ctttgtgccc ttcgtgcagc tgcgcccgct gagcgcgctg gctgaagctg 481 gagaggcggt gctgctgctg cgggaggggc tgctgcgccg cgtgcgttgc ctgcagctgg 541 ggtccccagg tccttgcccc gtggccgccg gccccgggcc cgcctccgtc tctggccttg 601 ccgcggggtc cggccgcgac tgcgtgctgc tgcaagaggg ctttctggcg cacaggggcc 661 gaccccacgt ctacctgcag cgcatccagc tcaacaaccc cacggagcgc gtgaccgcgc 721 tgcagactgt ggggcccact gccggcccag cccccaaggc cttcaccagt accctggaga 781 aggtcggaga ccatcagttc ctcctctact caggccggtc cccgcctacg cccactgggt 841 tggtgcacct ggtggtggtg gccgccaaga agctggtgaa ccgcctccaa gtggctccca 901 agacgcagct ggatgagacg gtgctgtggg tggtgcacgt ctctggcccc attaaccccc 961 aggtgcccaa aagcaaagca gccaaggagc tcaaggcgct gcaggacttg gcacggaagg 1021 aaatgctgga gctcttggag atgccagcgg cggagctgct tcaagaccac cagctcctct 1081 gggctcagct cttcagccca ggagtggaaa tgaagatcac tgacacccac aggccgtctg 1141 gcctcaccgt gaacctgacg ctctattaca tgctctcctg ctcgccagcc ccgctgctca 1201 gcccctccct gagccacagg aagcgagacc agatggagtc gacgctcaac tatgaagatc 1261 accgcttcag cgggcacgcc accatgcacg ccgagaacct gtggccgggg ctgctgtcct 1321 ccgtccagca gatcctgcag ctctctgacc tgtggaggct gaccctccag aagagtggct 1381 gcaaggggct ggtgaaggtg ggtgccccag gcatcctgca gggcatggtg ctcagcttcg 1441 gggggctgca gttcacagag aaccacctcc agttccaggc cgaccccgac gtgctgcaga 1501 acagctatgc attgcatggc atccgctaca agaacgacca tatcaacctg gccgtgctgg 1561 cggatgccga gggcaagccc taccTACACG AGTCCGTGGA GTCCCGTGGC CAGCCTGTCA 1621 AGATCTATGC CTGCAAGGCA GGCTGCCTGG ACGAGCCAGT GGAGCTGACC TCGGCGCCCA 1681 CGGGCCACAC CTTCTCGGTC ATGGTGACAC AGCCCATCAC GCCACTGCTC TACATCTCCA 1741 CCGACCTCAC ACACCTGCAG GACCTGCGGC ACACGCTGCA CCTCAAGGCC ATCCTGGCCC 1801 ATGATGAGCA CATGGCTCAG CAGGACCCCG GGCTGCCCTT CCTCTTCTGG TTCAGCTTGG 1861 CCTCCCTCAT CACCCTCTTC CACCTCTTCC TCTTCAAGCT CATCTACAAC GAGTACTGTG 1921 GGCCTGGAGC CAAGCCCCTC TGCAGGAGTA AGGAAGATCC CAGTGTCTTG CCAACTTTCT 1981 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2041 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2101 GTGGAAAGGA CGATCAATTT AGCCGGCGCC GGGTAACACG CGTTAAGTCg acaatcaacc 2161 tctggattac aaaatttgtg aaagatt