Construct: ORF TRCN0000481313
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004340.1_s317c1
- Derived from:
- ccsbBroadEn_09581
- DNA Barcode:
- ACTTTATGTAATGCCGTTCGTAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM199X (139231)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481313
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 139231 | FAM199X | family with sequence simila... | NM_207318.4 | 99.9% | 99.7% | 7G>A |
| 2 | human | 139231 | FAM199X | family with sequence simila... | XM_005262079.3 | 88.8% | 88.6% | 7G>A;867_868ins129 |
| 3 | mouse | 245622 | Fam199x | family with sequence simila... | NM_146261.2 | 92.7% | 97.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1230
- ORF length:
- 1164
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc caacgaggcc tcggccatca cttcctacga gaagtttcta acccccgagg 121 agcccttccc actcctggga cctcctcgcg gggtgggcac ctgcccgagc gaggagccgg 181 gctgcctgga catcagcgac ttcggctgcc agctgtcctc ctgccatcgc accgacccgc 241 tccaccgctt ccacaccaac aggtggaacc taacttcttg tggaacaagt gttgccagct 301 cagaaggcag tgaggagctg ttttcatctg tgtctgttgg agatcaagat gattgctatt 361 ccctgttaga tgatcaggac ttcacttctt ttgatttatt tcctgagggg agtgtctgca 421 gtgatgtctc ttcttctatt agcacttact gggattggtc agatagcgag tttgaatggc 481 agttaccagg cagtgacatt gccagtggga gtgatgtact ttctgatgtc atacccagta 541 ttccaagttc accttgcctg cttcctaaaa agaaaaacaa gcaccggaat ttagatgaac 601 tcccttggag tgcaatgaca aatgatgagc aggtggaata tattgagtat ctgagtcgga 661 aagtgagtac tgagatgggt cttcgggagc aacttgatat tattaagatc attgatcctt 721 ctgctcaaat ctcccctaca gacagtgagt ttattattga acttaactgt ctcacagatg 781 aaaaactgaa gcaggtcaga aactatatca aggaacatag cccTCGCCAA CGGCCTGCAA 841 GAGAGGCCTG GAAGAGAAGC AACTTTAGTT GTGCAAGCAC CAGTGGAGTG AGCGGTGCCA 901 GTGCCAGCGC CAGCAGCAGC AGTGCCAGCA TGGTCAGTTC TGCAAGCAGC AGTGGGTCCA 961 GTGTTGGAAA CTCTGCTTCA AACTCCAGTG CCAACATGAG TCGAGCACAC AGTGACAGCA 1021 ACCTGTCTGC AAGTGCAGCA GAGCGGATTC GGGATTCAAA AAAGCGATCC AAGCAGCGGA 1081 AGTTACAGCA GAAGGCCTTC CGCAAGAGGC AGCTGAAGGA GCAGAGGCAG GCCCGGAAGG 1141 AGAGGCTCAG TGGGCTCTTC CTTAACGAAG AGGTGCTGTC CTTGAAAGTG ACTGAGGAAG 1201 ACCATGAAGC AGATGTTGAT GTTTTGATGT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1261 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1321 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAACTTT 1381 ATGTAATGCC GTTCGTAATA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1441 tgaaagatt