Construct: ORF TRCN0000481331
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013118.1_s317c1
- Derived from:
- ccsbBroadEn_15892
- DNA Barcode:
- TTTCGGATGGACACTTGGAAGTAT
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM39A (55254)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481331
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55254 | TMEM39A | transmembrane protein 39A | XM_011512958.3 | 45.1% | 43.7% | (many diffs) |
| 2 | human | 55254 | TMEM39A | transmembrane protein 39A | XM_005247578.2 | 38.2% | 37.1% | (many diffs) |
| 3 | human | 55254 | TMEM39A | transmembrane protein 39A | NM_018266.3 | 29.5% | 28.6% | (many diffs) |
| 4 | human | 55254 | TMEM39A | transmembrane protein 39A | XM_006713687.2 | 29.5% | 28.6% | (many diffs) |
| 5 | human | 55254 | TMEM39A | transmembrane protein 39A | XM_017006788.2 | 28.6% | 25.7% | (many diffs) |
| 6 | human | 55254 | TMEM39A | transmembrane protein 39A | XR_001740197.2 | 9.6% | (many diffs) | |
| 7 | human | 55254 | TMEM39A | transmembrane protein 39A | XR_001740198.2 | 9% | (many diffs) | |
| 8 | human | 55254 | TMEM39A | transmembrane protein 39A | NR_073506.2 | 8.8% | 1_354del;783delG;793_4962del | |
| 9 | mouse | 67846 | Tmem39a | transmembrane protein 39a | XM_006522497.3 | 40.8% | 41.1% | (many diffs) |
| 10 | mouse | 67846 | Tmem39a | transmembrane protein 39a | NM_001205287.1 | 31% | 31.3% | (many diffs) |
| 11 | mouse | 67846 | Tmem39a | transmembrane protein 39a | NM_001205286.1 | 26.7% | 26.9% | (many diffs) |
| 12 | mouse | 67846 | Tmem39a | transmembrane protein 39a | NM_026407.3 | 26.7% | 26.9% | (many diffs) |
| 13 | mouse | 67846 | Tmem39a | transmembrane protein 39a | XR_384596.3 | 20% | (many diffs) | |
| 14 | mouse | 67846 | Tmem39a | transmembrane protein 39a | XR_001781854.1 | 17.9% | (many diffs) | |
| 15 | mouse | 67846 | Tmem39a | transmembrane protein 39a | XR_001781853.1 | 12.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 504
- ORF length:
- 438
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc cggtggaagg aggggcccta gtcggcaaca gctaagccgt tcagctttac 121 cttctttgCA GACTTTGGTT GGTGGAGGCT GTGGCAATGG AACAGGCTTG AGAAACAGGA 181 ATGGTAGTGC TATTGGCCTT CCAGTCCCAC CTATCACAGC CTTAATCACC CCAGGTCCTG 241 TTCGTCATTG CCAAATTCCT GACTTGCCTG TGGATGGGAG CCTACTCTTT GAATTCCTTT 301 TTTTCATCTA CCTGTTGGTT GCTCTTTTCA TTCAGTACAT CAACATTTAT AAAACAGTGT 361 GGTGGTATCC TTACAATCAT CCTGCTTCTT GTACATCACT GAATTTTCAT CTCATTGATT 421 ATCATCTGGC AGCATTCATC ACAGTGATGC TTGCGAGGAG GCTTGTATGG GCTCTCATCT 481 CAGAGGTCTG TGTTGTAAGC TCTGTCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA 541 GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT 601 TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATT TCGGATGGAC 661 ACTTGGAAGT ATACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga 721 tt