Construct: ORF TRCN0000481346

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018093.1_s317c1
Derived from:
ccsbBroadEn_01754
DNA Barcode:
CGCACCATGCACTAGAGCTTTTTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
UCK2 (7371)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481346
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7371 UCK2 uridine-cytidine kinase 2 NM_012474.5 100% 100%
2 human 7371 UCK2 uridine-cytidine kinase 2 NM_001363568.2 87.3% 87.4% (many diffs)
3 mouse 80914 Uck2 uridine-cytidine kinase 2 NM_030724.3 90.2% 98% (many diffs)
4 mouse 80914 Uck2 uridine-cytidine kinase 2 XM_006497040.3 78.7% 85.4% (many diffs)
5 mouse 80914 Uck2 uridine-cytidine kinase 2 XM_017312968.1 60.9% 62.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
849
ORF length:
783
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc cggggacagc gagcagaccc tgcagaacca ccagcagccc aacggcggcg
121agcccttcct tataggcgtc agcgggggaa cagctagcgg caagtcttcc gtgtgtgcta
181agatcgtgca gctcctgggg cagaatgagg tggactatcg ccagaagcag gtggtcatcc
241tgagccagga tagcttctac cgtgtcctta cctcggagca gaaggccaaa gccctgaagg
301gccagttcaa ctttgaccac ccggatgcct ttgacaatga actcattctc aaaacactca
361aagaaatcac tgaagggaaa acagtccaga tccccgtgta tgactttgtc tcccattccc
421ggaaggagga gacagttact gtctatcccg cagacgtggt gctctttgaa gggatcctgg
481ccTTCTACTC CCAGGAGGTA CGAGACCTGT TCCAGATGAA GCTTTTTGTG GATACAGATG
541CGGACACCCG GCTCTCACGC AGAGTATTAA GGGACATCAG CGAGAGAGGC AGGGATCTTG
601AGCAGATTTT ATCTCAGTAC ATTACGTTCG TCAAGCCTGC CTTTGAGGAA TTCTGCTTGC
661CAACAAAGAA GTATGCTGAT GTGATCATCC CTAGAGGTGC AGATAATCTG GTGGCCATCA
721ACCTCATCGT GCAGCACATC CAGGACATCC TGAATGGAGG GCCCTCCAAA CGGCAGACCA
781ATGGCTGTCT CAACGGCTAC ACCCCTTCAC GCAAGAGGCA GGCATCGGAG TCCAGCAGCA
841GGCCGCATTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
901CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
961TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACGCACC ATGCACTAGA GCTTTTTTAC
1021GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)