Construct: ORF TRCN0000481350
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015897.1_s317c1
- Derived from:
- ccsbBroadEn_15995
- DNA Barcode:
- CTAGCGAACAACCGTCGCTTTAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRM1 (79922)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481350
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79922 | MRM1 | mitochondrial rRNA methyltr... | NM_024864.5 | 43.8% | 43.6% | 1_594del;1049A>G |
| 2 | human | 79922 | MRM1 | mitochondrial rRNA methyltr... | XM_017025112.2 | 34% | 30.1% | (many diffs) |
| 3 | human | 79922 | MRM1 | mitochondrial rRNA methyltr... | XM_011525275.3 | 32.8% | 28.3% | (many diffs) |
| 4 | human | 79922 | MRM1 | mitochondrial rRNA methyltr... | XM_011525276.3 | 32.8% | 28.3% | (many diffs) |
| 5 | human | 79922 | MRM1 | mitochondrial rRNA methyltr... | XR_934554.2 | 14.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 531
- ORF length:
- 465
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGA CGTGTTCTCC ACTGATGACC TCACCGGATT TTTACAGACC AAAGCCCAGC 121 AGGGCTGGCT CGTGGCCGGC ACGGTGGGCT GCCCAAGCAC AGAGGATCCC CAGTCCTCCG 181 AGATCCCCAT CATGAGTTGC TTGGAGTTCC TCTGGGAACG GCCTACTCTC CTTGTGCTGG 241 GGAATGAGGG CTCAGGTCTA TCCCAGGAGG TGCAGGCCTC CTGCCAGCTT CTCCTCACCA 301 TCCTGCCCCG GCGCCAGCTG CCTCCTGGAC TTGAGTCCTT GAACGTCTCT GTGGCTGCAG 361 GAATTCTTCT TCACTCCATT TGCAGCCAGA GGAAGGGTTT CCCCACAGAG GGGGAGAGAA 421 GGCAGCTTCT CCAAGACCCC CAAGAACCCT CAGCCAGGTC TGAAGGGCTC AGCATGGCTC 481 AGCACCCAGG GCTGTCTTCA GGCCCAGAGA AAGAGAGGCG AAATGAGGGC TGCCCAACTT 541 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 601 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 661 CTTGTGGAAA GGACGACTAG CGAACAACCG TCGCTTTAAT ACGCGTTAAG TCgacaatca 721 acctctggat tacaaaattt gtgaaagatt