Construct: ORF TRCN0000481361
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006418.2_s317c1
- Derived from:
- ccsbBroadEn_04547
- DNA Barcode:
- GTACCGTCGAAGACCCCGAGGAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCED1B (91523)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481361
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91523 | PCED1B | PC-esterase domain containi... | NM_001281429.1 | 100% | 100% | |
| 2 | human | 91523 | PCED1B | PC-esterase domain containi... | NM_138371.3 | 100% | 100% | |
| 3 | human | 91523 | PCED1B | PC-esterase domain containi... | XM_005269224.5 | 100% | 100% | |
| 4 | human | 91523 | PCED1B | PC-esterase domain containi... | XM_011538978.2 | 100% | 100% | |
| 5 | human | 91523 | PCED1B | PC-esterase domain containi... | XM_017020207.1 | 100% | 100% | |
| 6 | human | 91523 | PCED1B | PC-esterase domain containi... | XM_017020208.1 | 100% | 100% | |
| 7 | human | 91523 | PCED1B | PC-esterase domain containi... | XM_017020209.1 | 100% | 100% | |
| 8 | human | 91523 | PCED1B | PC-esterase domain containi... | XM_017020210.1 | 100% | 100% | |
| 9 | human | 91523 | PCED1B | PC-esterase domain containi... | XM_017020211.1 | 100% | 100% | |
| 10 | human | 91523 | PCED1B | PC-esterase domain containi... | XM_017020212.1 | 100% | 100% | |
| 11 | human | 91523 | PCED1B | PC-esterase domain containi... | XM_017020213.1 | 100% | 100% | |
| 12 | human | 91523 | PCED1B | PC-esterase domain containi... | XM_017020214.1 | 100% | 100% | |
| 13 | human | 91523 | PCED1B | PC-esterase domain containi... | XM_017020215.1 | 100% | 100% | |
| 14 | human | 91523 | PCED1B | PC-esterase domain containi... | XM_017020216.1 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1362
- ORF length:
- 1296
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat ccttctgcgg gcctccgaag tgcggcagct gcttcacaat aagttcgtgg 121 tcatcctggg ggactctgtg catagggcag tatacaagga cctggtgctt ctgctgcaga 181 aggaccgcct gctcactccc gggcagctta gagcaagggg ggagctgaac ttcgaacaag 241 atgagctggt ggacggaggc cagcggggcc acatgcacaa cggccttaac taccgtgagg 301 tccgcgagtt ccgctccgac caccatctgg tacgttttta cttcctcacc cgcgtgtact 361 ccgattacct ccagaccatc ttgaaagagc tgcagtcggg cgagcacgcc cccgacctgg 421 tcatcatgaa ttcctgcctc tgggacatct ccaggtatgg tccgaactcc tggagaagct 481 acctggagaa cctggagaac ctgttccagt gcctgggcca ggtgctgccc gagtcttgcc 541 tcctggtgtg gaacacggcc atgcctgtgg gcgaggaagt caccgggggt tttcttccgc 601 ccaagctccg gcggcagaag gccaccttcc tgaaaaacga agtggtcaaa gccaacttcc 661 acagcgccac cgaggcacgt aaacataact tcgatgtact ggacttgcat ttccacttcc 721 gccacgcgag ggagaacctg cactgggacg gggtgcactg gaatggacgt gtgcaccgct 781 gcctctccca gctgctgctg gcccacgtgg ccgacgcctg gggtgtggag ctgccccacc 841 gccaccccgt gggcgagtgg atcaagaaga aaaaacctgg cccgagagtc gaagggccgc 901 cccaggccaa cagaaatcac ccggccttac ctctgtcccc acCCTTACCT TCCCCCACAT 961 ACCGCCCCCT GCTTGGGTTC CCACCCCAGC GCTTGCCGCT GCTCCCGCTC CTGTCCCCAC 1021 AGCCTCCTCC TCCCATTCTC CATCACCAGG GAATGCCCCG GTTCCCACAG GGTCCCCCAG 1081 ATGCCTGTTT TTCCTCAGAC CATACTTTCC AGTCGGATCA ATTCTATTGC CATTCAGATG 1141 TCCCCTCATC AGCCCATGCA GGTTTCTTCG TCGAAGACAA TTTTATGGTT GGTCCTCAGC 1201 TGCCTATGCC CTTCTTCCCC ACACCCCGTT ATCAGCGGCC TGCCCCAGTG GTACATAGGG 1261 GTTTTGGCAG GTATCGTCCC CGTGGCCCCT ATACGCCCTG GGGACAGCGG CCTCGACCTT 1321 CAAAGAGAAG GGCCCCAGCC AATCCTGAGC CAAGGCCTCA ATACCCAACT TTCTTGTACA 1381 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1441 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1501 AGGACGAGTA CCGTCGAAGA CCCCGAGGAG CACGCGTTAA GTCgacaatc aacctctgga 1561 ttacaaaatt tgtgaaagat t