Construct: ORF TRCN0000481370
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000379.1_s317c1
- Derived from:
- ccsbBroadEn_12415
- DNA Barcode:
- TGAACCCTTGAATGACGCTTCCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF250 (58500)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481370
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 58500 | ZNF250 | zinc finger protein 250 | NM_001109689.4 | 91.8% | 91.8% | 1_135del |
| 2 | human | 58500 | ZNF250 | zinc finger protein 250 | NM_001363100.2 | 91.8% | 91.8% | 1_135del |
| 3 | human | 58500 | ZNF250 | zinc finger protein 250 | NM_001363101.2 | 91.8% | 91.8% | 1_135del |
| 4 | human | 58500 | ZNF250 | zinc finger protein 250 | NM_001363102.2 | 91.8% | 91.8% | 1_135del |
| 5 | human | 58500 | ZNF250 | zinc finger protein 250 | NM_001363103.2 | 91.8% | 91.8% | 1_135del |
| 6 | human | 58500 | ZNF250 | zinc finger protein 250 | NM_001363098.2 | 91% | 91% | 1_150del |
| 7 | human | 58500 | ZNF250 | zinc finger protein 250 | NM_001363099.2 | 91% | 91% | 1_150del |
| 8 | human | 58500 | ZNF250 | zinc finger protein 250 | NM_021061.5 | 91% | 91% | 1_150del |
| 9 | human | 58500 | ZNF250 | zinc finger protein 250 | XM_024447220.1 | 91% | 91% | 1_150del |
| 10 | human | 58500 | ZNF250 | zinc finger protein 250 | XM_024447221.1 | 91% | 91% | 1_150del |
| 11 | human | 58500 | ZNF250 | zinc finger protein 250 | NM_001363105.2 | 88.1% | 88.1% | 1_135del;282_283ins63 |
| 12 | human | 58500 | ZNF250 | zinc finger protein 250 | NM_001363106.2 | 88.1% | 88.1% | 1_135del;282_283ins63 |
| 13 | human | 58500 | ZNF250 | zinc finger protein 250 | NM_001363107.2 | 88.1% | 88.1% | 1_135del;282_283ins63 |
| 14 | human | 58500 | ZNF250 | zinc finger protein 250 | NM_001363104.2 | 87.3% | 87.3% | 1_150del;297_298ins63 |
| 15 | mouse | 239546 | Zfp647 | zinc finger protein 647 | NM_001168276.1 | 72.8% | 76.2% | (many diffs) |
| 16 | mouse | 239546 | Zfp647 | zinc finger protein 647 | NM_172817.3 | 72.8% | 76.2% | (many diffs) |
| 17 | mouse | 239546 | Zfp647 | zinc finger protein 647 | XM_006520928.3 | 72.8% | 76.2% | (many diffs) |
| 18 | mouse | 239546 | Zfp647 | zinc finger protein 647 | XM_006520929.3 | 72.8% | 76.2% | (many diffs) |
| 19 | mouse | 239546 | Zfp647 | zinc finger protein 647 | XM_011245617.2 | 72.8% | 76.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1596
- ORF length:
- 1530
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat ggaaacctat gggaatgtag tctcattggg acttccagga tccaagcctg 121 acataatctc ccagctggag cgaggggaag atccctgggt cctggacagg aagggggcta 181 agaagagcca gggcctgtgg agtgactact cagacaacct caaatatgac cacactacag 241 cctgtacaca acaagacagt ttatcttgtc catgggaatg tgaaaccaag ggagagagtc 301 aaaatacaga cttgagtccg aagccattaa tttcagagca aacagtgatt ctggggaaaa 361 cacccttggg gaggattgat caagaaaata atgaaacaaa gcaaagcttc tgtctgagtc 421 caaactctgt tgaccaccgt gaagttcagg tcttaagcca aagcatgcca ctcactccgc 481 accaggcagt gcctagtgga gagaggccct acatgtgtgt tgagtgtggg aagtgctttg 541 gccggagttc ccacctcctt cagcatcagc gtatccacac tggagagaag ccctatgtgt 601 gcagtgtatg tgggaaggcc ttcagccaga gctcagtcct tagtaaacac aggagaattc 661 acacaggtga gaagccctat gagtgtaatg agtgtggaaa agcctttaga gtgagctcag 721 atcttgctca gcatcacaag atacatacag gagagaagcc tcacgaatgt cttgagtgtc 781 ggaaagcctt cactcaactc tcacatctca ttcagcacca gcggatccac acgggagaaa 841 ggccatatgt gtgtccgttg tgtgggaaag ccttcaacca tagcactgtt ctgcggagcc 901 accagagggt acacactggg gagaagcctc acaggtgcaa tgagtgtggg aaaaccttca 961 gtgtgaagag gacactgctg cagcaccaga ggatccacac cggggagaag ccctacacgt 1021 gcagcgagtg tgggaaggcc ttcagcgacc gctcagtcct cattcagcac cacaacgtgc 1081 acaccgggga gaagccctat gagtgcagtg agtgtgggaa gaccTTCAGC CACCGCTCCA 1141 CACTGATGAA TCACGAGCGG ATCCACACCG AGGAAAAGCC CTATGCATGC TACGAATGTG 1201 GGAAGGCCTT CGTTCAGCAC TCACACCTGA TCCAGCACCA GAGAGTCCAC ACTGGGGAGA 1261 AGCCCTATGT GTGTGGTGAA TGTGGGCACG CCTTCAGTGC ACGCCGGTCT CTGATCCAGC 1321 ATGAGAGAAT CCACACAGGT GAAAAGCCCT TCCAGTGCAC AGAATGTGGC AAAGCCTTCA 1381 GCCTGAAAGC AACTCTGATT GTGCACCTGA GGACCCACAC GGGCGAGAAG CCATATGAGT 1441 GCAATAGCTG CGGGAAGGCC TTCAGCCAGT ACTCAGTGCT CATCCAGCAC CAGCGGATCC 1501 ACACAGGCGA GAAGCCCTAT GAGTGCGGGG AGTGTGGGCG TGCCTTCAAC CAGCATGGCC 1561 ACCTAATCCA GCACCAGAAA GTGCACAGAA AGTTGTGCCC AACTTTCTTG TACAAAGTGG 1621 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1681 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1741 ATGAACCCTT GAATGACGCT TCCCCACGCG TTAAGTCgac aatcaacctc tggattacaa 1801 aatttgtgaa agatt